Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer

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  • معلومة اضافية
    • Affiliation:
      a Department of Surgery, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, United States
      b Department of Epidemiology and Statistics, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, United States
    • Keywords:
      Pancreatic adenocarcinoma
      Oncolytic viral therapy
      Herpes simplex virus
      Peritoneal fluid cytology
      Diagnostic laparoscopy
    • Abstract:
      Introduction Patients with peritoneal dissemination of pancreatic adenocarcinoma do not benefit from surgical resection, but radiologic and cytologic detection of peritoneal cancer lack sensitivity. This trial sought to determine if an oncolytic virus may be used as a diagnostic agent to detect peritoneal cancer.
    • Abstract:
      Results Of 82 patients entered in this trial, 12 (15%) had positive cytology and 50 (61%) had virally-mediated eGFP positive cells in peritoneal washings. All cytology-positive patients were also eGFP positive. HSV-mediated fluorescence detection had sensitivities of 94% and 100% for detection of any and peritoneal metastatic disease; respectively. Median recurrence free and disease specific survival were 6.5 and 18.3months for eGFP positive patients, versus 12.2 and 36.2months for eGFP negative patients (P=0.01 and 0.19); respectively.
    • Abstract:
      Conclusions A genetically modified HSV can be used as a highly sensitive diagnostic agent for detection of micro-metastatic disease in patients with pancreatic adenocarcinoma and may improve patient selection for surgery.
    • Abstract:
      @@@@Highlights •Oncolytic virus-mediated fluorescence is a sensitive assay for detection of cancer cells in peritoneal fluid.•Pancreatic cancer patients with eGFP-positive cells in peritoneal washings had a poor prognosis following surgery.Pancreatic cancer is an aggressive disease. Even with complete surgical removal of a pancreatic tumor, recurrence is common. Patients with microscopic spread of cancer cells into the abdomen, or peritoneum, do not benefit from surgery. Current methods of detection of this kind of spread are not very sensitive. This study utilized a virus that specifically infects cancer cells and expresses a green fluorescent protein within them to detect peritoneal disease. Viral fluorescence was more sensitive than standard methods for detecting peritoneal disease and may help to identify which patients with pancreas cancer will benefit from surgery.
    • ISSN:
      2352-3964
    • Accession Number:
      10.1016/j.ebiom.2016.03.043
    • Accession Number:
      S2352396416301293
    • Copyright:
      Published by Elsevier B.V.
  • Citations
    • ABNT:
      KELLY, K. J. et al. Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer. EBioMedicine, [s. l.], v. 7, p. 94–99, 2016. Disponível em: . Acesso em: 15 nov. 2019.
    • AMA:
      Kelly KJ, Wong J, Gönen M, et al. Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer. EBioMedicine. 2016;7:94-99. doi:10.1016/j.ebiom.2016.03.043.
    • APA:
      Kelly, K. J., Wong, J., Gönen, M., Allen, P., Brennan, M., Coit, D., & Fong, Y. (2016). Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer. EBioMedicine, 7, 94–99. https://doi.org/10.1016/j.ebiom.2016.03.043
    • Chicago/Turabian: Author-Date:
      Kelly, Kaitlyn J., Joyce Wong, Mithat Gönen, Peter Allen, Murray Brennan, Daniel Coit, and Yuman Fong. 2016. “Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer.” EBioMedicine 7 (May): 94–99. doi:10.1016/j.ebiom.2016.03.043.
    • Harvard:
      Kelly, K. J. et al. (2016) ‘Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer’, EBioMedicine, 7, pp. 94–99. doi: 10.1016/j.ebiom.2016.03.043.
    • Harvard: Australian:
      Kelly, KJ, Wong, J, Gönen, M, Allen, P, Brennan, M, Coit, D & Fong, Y 2016, ‘Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer’, EBioMedicine, vol. 7, pp. 94–99, viewed 15 November 2019, .
    • MLA:
      Kelly, Kaitlyn J., et al. “Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer.” EBioMedicine, vol. 7, May 2016, pp. 94–99. EBSCOhost, doi:10.1016/j.ebiom.2016.03.043.
    • Chicago/Turabian: Humanities:
      Kelly, Kaitlyn J., Joyce Wong, Mithat Gönen, Peter Allen, Murray Brennan, Daniel Coit, and Yuman Fong. “Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer.” EBioMedicine 7 (May 1, 2016): 94–99. doi:10.1016/j.ebiom.2016.03.043.
    • Vancouver/ICMJE:
      Kelly KJ, Wong J, Gönen M, Allen P, Brennan M, Coit D, et al. Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer. EBioMedicine [Internet]. 2016 May 1 [cited 2019 Nov 15];7:94–9. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edselp&AN=S2352396416301293&custid=s8280428