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Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay.

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  • معلومة اضافية
    • Publication Information:
      Public Library of Science (PLoS), 2020.
    • Publication Date:
      2020
    • Collection:
      LCC:Medicine
      LCC:Science
    • Abstract:
      The majority of congenital cytomegalovirus (cCMV) infections are asymptomatic at birth and therefore not diagnosed. Approximately 10-15% of these infants develop late-onset hearing loss and other developmental disorders. Implementation of a universal screening approach at birth may allow early initiation of symptomatic interventions due to a closer follow-up of infants at risk and offers the opportunity to consider treatment of late-onset disease. Real-time PCR assays for the detection of CMV DNA in buccal swab samples demonstrated feasibility and good clinical sensitivity in comparison to a rapid culture screening assay. Because most cCMV infections remain asymptomatic, a universal screening assay that stratifies CMV infected infants according to low and high risk of late-onset cCMV disease could limit the parental anxiety and reduce follow-up costs. We therefore developed and characterized a screening algorithm based on a highly-sensitive quantitative real-time PCR assay that is compatible with centralized testing of samples from universal screening and allows to determine CMV DNA load of saliva samples either as International Units (IU)/ml saliva or IU/105 cell equivalents. 18 of 34 saliva samples of newborns that tested positively by the screening algorithm were confirmed by detection of CMV DNA in blood and/or urine samples obtained during the first weeks of life. All screening samples that could not be confirmed had viral loads of
    • File Description:
      electronic resource
    • ISSN:
      1932-6203
    • Relation:
      https://doaj.org/toc/1932-6203
    • Accession Number:
      10.1371/journal.pone.0227143
    • Rights:
      Journal Licence: CC BY
    • Accession Number:
      edsdoj.f0b10f295a074cc48c2db7e462e32cfe
  • Citations
    • ABNT:
      ANGELA NAGEL et al. Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay. PLoS ONE, [s. l.], n. 1, p. e0227143, 2020. DOI 10.1371/journal.pone.0227143. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsdoj&AN=edsdoj.f0b10f295a074cc48c2db7e462e32cfe&custid=s8280428. Acesso em: 5 abr. 2020.
    • AMA:
      Angela Nagel, Emmanouela Dimitrakopoulou, Norbert Teig, et al. Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay. PLoS ONE. 2020;(1):e0227143. doi:10.1371/journal.pone.0227143.
    • APA:
      Angela Nagel, Emmanouela Dimitrakopoulou, Norbert Teig, Peter Kern, Thomas Lücke, Dariusz Michna, Klaus Korn, Philipp Steininger, Khalid Shahada, Katrin Neumann, & Klaus Überla. (2020). Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay. PLoS ONE, 1, e0227143. https://doi.org/10.1371/journal.pone.0227143
    • Chicago/Turabian: Author-Date:
      Angela Nagel, Emmanouela Dimitrakopoulou, Norbert Teig, Peter Kern, Thomas Lücke, Dariusz Michna, Klaus Korn, et al. 2020. “Characterization of a Universal Screening Approach for Congenital CMV Infection Based on a Highly-Sensitive, Quantitative, Multiplex Real-Time PCR Assay.” PLoS ONE, no. 1: e0227143. doi:10.1371/journal.pone.0227143.
    • Harvard:
      Angela Nagel et al. (2020) ‘Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay’, PLoS ONE, (1), p. e0227143. doi: 10.1371/journal.pone.0227143.
    • Harvard: Australian:
      Angela Nagel, Emmanouela Dimitrakopoulou, Norbert Teig, Peter Kern, Thomas Lücke, Dariusz Michna, Klaus Korn, Philipp Steininger, Khalid Shahada, Katrin Neumann & Klaus Überla 2020, ‘Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay’, PLoS ONE, no. 1, p. e0227143, viewed 5 April 2020, .
    • MLA:
      Angela Nagel, et al. “Characterization of a Universal Screening Approach for Congenital CMV Infection Based on a Highly-Sensitive, Quantitative, Multiplex Real-Time PCR Assay.” PLoS ONE, no. 1, 2020, p. e0227143. EBSCOhost, doi:10.1371/journal.pone.0227143.
    • Chicago/Turabian: Humanities:
      Angela Nagel, Emmanouela Dimitrakopoulou, Norbert Teig, Peter Kern, Thomas Lücke, Dariusz Michna, Klaus Korn, et al. “Characterization of a Universal Screening Approach for Congenital CMV Infection Based on a Highly-Sensitive, Quantitative, Multiplex Real-Time PCR Assay.” PLoS ONE, no. 1 (2020): e0227143. doi:10.1371/journal.pone.0227143.
    • Vancouver/ICMJE:
      Angela Nagel, Emmanouela Dimitrakopoulou, Norbert Teig, Peter Kern, Thomas Lücke, Dariusz Michna, et al. Characterization of a universal screening approach for congenital CMV infection based on a highly-sensitive, quantitative, multiplex real-time PCR assay. PLoS ONE [Internet]. 2020 [cited 2020 Apr 5];(1):e0227143. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsdoj&AN=edsdoj.f0b10f295a074cc48c2db7e462e32cfe&custid=s8280428