Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion

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  • معلومة اضافية
    • Publication Information:
      BMC, 2019.
    • Publication Date:
      2019
    • Collection:
      LCC:Infectious and parasitic diseases
    • Abstract:
      Abstract Background Elimination of malaria depends on mastering transmission and understanding the biological basis of Plasmodium infection in the vector. The first mosquito organ to interact with the parasite is the midgut and its transcriptomic characterization during infection can reveal effective antiplasmodial responses able to limit the survival of the parasite. The vector response to Plasmodium vivax is not fully characterized, and its specificities when compared with other malaria parasites can be of fundamental interest for specific control measures. Methods Experimental infections were performed using a membrane-feeding device. Three groups were used: P. vivax-blood-fed, blood-fed on inactivated gametocytes, and unfed mosquitoes. Twenty-four hours after feeding, the mosquitoes were dissected and the midgut collected for transcriptomic analysis using RNAseq. Nine cDNA libraries were generated and sequenced on an Illumina HiSeq2500. Readings were checked for quality control and analysed using the Trinity platform for de novo transcriptome assembly. Transcript quantification was performed and the transcriptome was functionally annotated. Differential expression gene analysis was carried out. The role of the identified mechanisms was further explored using functional approaches. Results Forty-nine genes were identified as being differentially expressed with P. vivax infection: 34 were upregulated and 15 were downregulated. Half of the P. vivax-related differentially expressed genes could be related to autophagy; therefore, the effect of the known inhibitor (wortmannin) and activator (spermidine) was tested on the infection outcome. Autophagic activation significantly reduced the intensity and prevalence of infection. This was associated with transcription alterations of the autophagy regulating genes Beclin, DRAM and Apg8. Conclusions Our data indicate that P. vivax invasion of An. aquasalis midgut epithelium triggers an autophagic response and its activation reduces infection. This suggests a novel mechanism that mosquitoes can use to fight Plasmodium infection.
    • File Description:
      electronic resource
    • ISSN:
      1756-3305
      51514672
    • Relation:
      http://link.springer.com/article/10.1186/s13071-019-3506-8; https://doaj.org/toc/1756-3305
    • Accession Number:
      10.1186/s13071-019-3506-8
    • Rights:
      Journal Licence: CC BY
    • Accession Number:
      edsdoj.42d173fb51514672966f562e405d86a0
  • Citations
    • ABNT:
      ROSA AMÉLIA GONÇALVES SANTANA et al. Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion. Parasites & Vectors, [s. l.], n. 1, p. 1, 2019. DOI 10.1186/s13071-019-3506-8. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsdoj&AN=edsdoj.42d173fb51514672966f562e405d86a0&custid=s8280428. Acesso em: 13 dez. 2019.
    • AMA:
      Rosa Amélia Gonçalves Santana, Maurício Costa Oliveira, Iria Cabral, et al. Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion. Parasites & Vectors. 2019;(1):1. doi:10.1186/s13071-019-3506-8.
    • APA:
      Rosa Amélia Gonçalves Santana, Maurício Costa Oliveira, Iria Cabral, Rubens Celso Andrade Silva Junior, Débora Raysa Teixeira de Sousa, Lucas Ferreira, … Henrique Silveira. (2019). Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion. Parasites & Vectors, (1), 1. https://doi.org/10.1186/s13071-019-3506-8
    • Chicago/Turabian: Author-Date:
      Rosa Amélia Gonçalves Santana, Maurício Costa Oliveira, Iria Cabral, Rubens Celso Andrade Silva Junior, Débora Raysa Teixeira de Sousa, Lucas Ferreira, Marcus Vinícius Guimarães Lacerda, et al. 2019. “Anopheles Aquasalis Transcriptome Reveals Autophagic Responses to Plasmodium Vivax Midgut Invasion.” Parasites & Vectors, no. 1: 1. doi:10.1186/s13071-019-3506-8.
    • Harvard:
      Rosa Amélia Gonçalves Santana et al. (2019) ‘Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion’, Parasites & Vectors, (1), p. 1. doi: 10.1186/s13071-019-3506-8.
    • Harvard: Australian:
      Rosa Amélia Gonçalves Santana, Maurício Costa Oliveira, Iria Cabral, Rubens Celso Andrade Silva Junior, Débora Raysa Teixeira de Sousa, Lucas Ferreira, Marcus Vinícius Guimarães Lacerda, Wuelton Marcelo Monteiro, Patrícia Abrantes, Maria das Graças Vale Barbosa Guerra & Henrique Silveira 2019, ‘Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion’, Parasites & Vectors, no. 1, p. 1, viewed 13 December 2019, .
    • MLA:
      Rosa Amélia Gonçalves Santana, et al. “Anopheles Aquasalis Transcriptome Reveals Autophagic Responses to Plasmodium Vivax Midgut Invasion.” Parasites & Vectors, no. 1, 2019, p. 1. EBSCOhost, doi:10.1186/s13071-019-3506-8.
    • Chicago/Turabian: Humanities:
      Rosa Amélia Gonçalves Santana, Maurício Costa Oliveira, Iria Cabral, Rubens Celso Andrade Silva Junior, Débora Raysa Teixeira de Sousa, Lucas Ferreira, Marcus Vinícius Guimarães Lacerda, et al. “Anopheles Aquasalis Transcriptome Reveals Autophagic Responses to Plasmodium Vivax Midgut Invasion.” Parasites & Vectors, no. 1 (2019): 1. doi:10.1186/s13071-019-3506-8.
    • Vancouver/ICMJE:
      Rosa Amélia Gonçalves Santana, Maurício Costa Oliveira, Iria Cabral, Rubens Celso Andrade Silva Junior, Débora Raysa Teixeira de Sousa, Lucas Ferreira, et al. Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion. Parasites & Vectors [Internet]. 2019 [cited 2019 Dec 13];(1):1. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsdoj&AN=edsdoj.42d173fb51514672966f562e405d86a0&custid=s8280428