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Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the innate immune system.

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  • معلومة اضافية
    • Publication Information:
      Public Library of Science (PLoS), 2019.
    • Publication Date:
      2019
    • Collection:
      DOAJ-Articles_enriched
      DOAJ-Articles
    • Abstract:
      Enterococcus faecalis is an opportunistic pathogen with an intrinsically high resistance to lysozyme, a key effector of the innate immune system. This high level of resistance requires a complex network of transcriptional regulators and several genes (oatA, pgdA, dltA and sigV) acting synergistically to inhibit both the enzymatic and cationic antimicrobial peptide activities of lysozyme. We sought to identify novel genes modulating E. faecalis resistance to lysozyme. Random transposon mutagenesis carried out in the quadruple oatA/pgdA/dltA/sigV mutant led to the identification of several independent insertions clustered on the chromosome. These mutations were located in a locus referred to as the enterococcal polysaccharide antigen (EPA) variable region located downstream of the highly conserved epaA-epaR genes proposed to encode a core synthetic machinery. The epa variable region was previously proposed to be responsible for EPA decorations, but the role of this locus remains largely unknown. Here, we show that EPA decoration contributes to resistance towards charged antimicrobials and underpins virulence in the zebrafish model of infection by conferring resistance to phagocytosis. Collectively, our results indicate that the production of the EPA rhamnopolysaccharide backbone is not sufficient to promote E. faecalis infections and reveal an essential role of the modification of this surface polymer for enterococcal pathogenesis.
    • Accession Number:
      pmc: PMC6497286
      pmid: 31048927
    • ISSN:
      1553-7374
      1553-7366
    • Accession Number:
      10.1371/journal.ppat.1007730
    • Accession Number:
      edsair.dedup.wf.001..d05be0d7f138b682944cd42c87e14c78
  • Citations
    • ABNT:
      ROBERT E SMITH et al. Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the innate immune system. PLoS Pathogens, [s. l.], v. 15, 2019. DOI 10.1371/journal.ppat.1007730. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsair&AN=edsair.dedup.wf.001..d05be0d7f138b682944cd42c87e14c78&custid=s8280428. Acesso em: 24 maio. 2020.
    • AMA:
      Robert E Smith, Bartłomiej Salamaga, Piotr Szkuta, et al. Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the innate immune system. PLoS Pathogens. 2019;15. doi:10.1371/journal.ppat.1007730.
    • APA:
      Robert E Smith, Bartłomiej Salamaga, Piotr Szkuta, Natalia Hajdamowicz, Tomasz K Prajsnar, Gregory S Bulmer, Thierry Fontaine, Justyna Kołodziejczyk, Jean-Marie Herry, Andrea M Hounslow, Mike P Williamson, Pascale Serror, & Stéphane Mesnage. (2019). Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the innate immune system. PLoS Pathogens, 15. https://doi.org/10.1371/journal.ppat.1007730
    • Chicago/Turabian: Author-Date:
      Robert E Smith, Bartłomiej Salamaga, Piotr Szkuta, Natalia Hajdamowicz, Tomasz K Prajsnar, Gregory S Bulmer, Thierry Fontaine, et al. 2019. “Decoration of the Enterococcal Polysaccharide Antigen EPA Is Essential for Virulence, Cell Surface Charge and Interaction with Effectors of the Innate Immune System.” PLoS Pathogens 15 (May). doi:10.1371/journal.ppat.1007730.
    • Harvard:
      Robert E Smith et al. (2019) ‘Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the innate immune system’, PLoS Pathogens, 15. doi: 10.1371/journal.ppat.1007730.
    • Harvard: Australian:
      Robert E Smith, Bartłomiej Salamaga, Piotr Szkuta, Natalia Hajdamowicz, Tomasz K Prajsnar, Gregory S Bulmer, Thierry Fontaine, Justyna Kołodziejczyk, Jean-Marie Herry, Andrea M Hounslow, Mike P Williamson, Pascale Serror & Stéphane Mesnage 2019, ‘Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the innate immune system’, PLoS Pathogens, vol. 15, viewed 24 May 2020, .
    • MLA:
      Robert E Smith, et al. “Decoration of the Enterococcal Polysaccharide Antigen EPA Is Essential for Virulence, Cell Surface Charge and Interaction with Effectors of the Innate Immune System.” PLoS Pathogens, vol. 15, May 2019. EBSCOhost, doi:10.1371/journal.ppat.1007730.
    • Chicago/Turabian: Humanities:
      Robert E Smith, Bartłomiej Salamaga, Piotr Szkuta, Natalia Hajdamowicz, Tomasz K Prajsnar, Gregory S Bulmer, Thierry Fontaine, et al. “Decoration of the Enterococcal Polysaccharide Antigen EPA Is Essential for Virulence, Cell Surface Charge and Interaction with Effectors of the Innate Immune System.” PLoS Pathogens 15 (May 1, 2019). doi:10.1371/journal.ppat.1007730.
    • Vancouver/ICMJE:
      Robert E Smith, Bartłomiej Salamaga, Piotr Szkuta, Natalia Hajdamowicz, Tomasz K Prajsnar, Gregory S Bulmer, et al. Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the innate immune system. PLoS Pathogens [Internet]. 2019 May 1 [cited 2020 May 24];15. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsair&AN=edsair.dedup.wf.001..d05be0d7f138b682944cd42c87e14c78&custid=s8280428