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Glucose administration downregulates lipoprotein lipase activity in vivo: a study using repeated intravenous fat tolerance test.

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  • Author(s): Jindrichová E;Jindrichová E; Kratochvílová S; Kovár J
  • Source:
    Physiological research [Physiol Res] 2007; Vol. 56 (2), pp. 175-81. Date of Electronic Publication: 2006 Mar 23.
  • Publication Type:
    Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • معلومة اضافية
    • Source:
      Publisher: Published for the Institute of Physiology of the Czechoslovak Academy of Sciences by Academia, Pub. House of the Academy Country of Publication: Czech Republic NLM ID: 9112413 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0862-8408 (Print) Linking ISSN: 08628408 NLM ISO Abbreviation: Physiol Res Subsets: MEDLINE
    • Publication Information:
      Original Publication: Praha : Published for the Institute of Physiology of the Czechoslovak Academy of Sciences by Academia, Pub. House of the Academy
    • Subject Terms:
    • Abstract:
      Lipoprotein lipase (LPL) is a key factor determining the clearance of triglycerides from the circulation. The enzyme activity is tissue-specifically regulated by insulin, but it is not clear yet how insulin regulates the total LPL activity in the circulation. To answer such question, we measured LPL activity using the intravenous fat tolerance test (IVFTT) that was carried out 1 h before as well as 2 h and 4 h after oral administration of glucose (75 g) in eleven healthy male volunteers. In control experiments, no glucose was given to the subjects. Glucose administration resulted in an expected increase in plasma glucose and insulin and in a suppression of non-esterified fatty acid concentration. The LPL activity assessed in IVFTT as a k(2) rate constant did not change in control experiments and decreased to 78 % and 73 % of baseline values 2 h and 4 h after glucose administration, respectively (p=0.01). Similarly, LPL activity measured in the plasma after intravenous injection of heparin at the end of the experiments was 16 % lower (p<0.05) after glucose administration. In conclusion, LPL activity is already downregulated in vivo 2 h after glucose administration. The results of our study indicate that repeated IVFTT is a promising approach for studying acute changes in LPL activity.
    • Accession Number:
      0 (Blood Glucose)
      0 (Fat Emulsions, Intravenous)
      0 (Fatty Acids, Nonesterified)
      0 (Insulin)
      0 (Triglycerides)
      EC 3.1.1.34 (Lipoprotein Lipase)
    • Publication Date:
      Date Created: 20060325 Date Completed: 20070801 Latest Revision: 20191210
    • Publication Date:
      20210210
    • Accession Number:
      16555944