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Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects.

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  • معلومة اضافية
    • Subject Terms:
    • Author-Supplied Keywords:
      cartilage particles
      cell sheet
      human amniotic mesenchymal stem cells
      osteochondral defect
    • NAICS/Industry Codes:
      112930 Fur-Bearing Animal and Rabbit Production
      311615 Poultry Processing
    • Abstract:
      Background: Human amniotic mesenchymal stem cells (hAMSCs) are being widely applied in various fields. Therefore, hAMSCs represent a promising candidate to facilitate cartilage regeneration. Nonetheless, no studies have investigated the application of hAMSC sheets to repair cartilage defects in vivo. Purpose: To evaluate hAMSC sheets encapsulating cartilage particles to promote repair of rabbit osteochondral defects. Study Design: Controlled laboratory study. Methods: hAMSC sheets were constructed with passage 3 hAMSCs. The phenotypic and structural characteristics of hAMSC sheets were evaluated by flow cytometry and scanning electron microscopy, respectively. The potential for chondrogenic differentiation of hAMSC sheets was assessed by cartilage-specific marker staining, immunohistochemistry, and mRNA and protein expression (SOX9, COLII, and ACAN). Osteochondral defects (diameter, 3.5 mm; depth, 3 mm) were created in the left patellar grooves of 20 New Zealand White rabbits (female or male). The defects were treated with hAMSC sheet/cartilage particles (n = 5), cartilage particles (n = 5), hAMSC sheets (n = 5), or fibrin glue (n = 5). Macroscopic and histological evaluations of the regenerated tissue were conducted after 3 months. The survival time and differentiation of transplanted hAMSCs in the defect area were evaluated by immunofluorescence. Results: hAMSC sheets had a multilayered structure, with cells stacked layer by layer. Importantly, hAMSC sheets highly expressed phenotypic markers of mesenchymal stem cells. Cartilage-specific marker staining and immunohistochemistry were positive, and mRNA and protein expression was higher in the chondrogenically induced hAMSC sheet group than in the hAMSC sheet group (P <.05). hAMSC sheet/cartilage particles formed a large amount of hyaline-like cartilage in the defect area. In addition, macroscopic and histological scores were significantly higher than those in the other groups. Integration with surrounding normal cartilage and subchondral bone regeneration in the hAMSC sheet/cartilage particles group were better when compared with the other groups. A large number of human nuclear-specific antigen-positive cells were observed in the defect area of hAMSC sheet/cartilage particles and hAMSC sheet groups. Moreover, some positive cells expressed SOX9. Conclusion: hAMSC sheets encapsulating cartilage particles facilitate osteochondral defect repair. Clinical Relevance: Delivery of cells in the form of a cell sheet in conjunction with cartilage particles provides a novel approach for cell-based cartilage regeneration. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • Author Affiliations:
      1First Department of Orthopedics, The Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou Province, China
      2Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, Beijing, China
      3Hospital of Stomatology, Zunyi Medical University, Zunyi, China
      4Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China
    • Full Text Word Count:
      6969
    • ISSN:
      0363-5465
    • Accession Number:
      10.1177/0363546519897912
    • Accession Number:
      141996871
  • Citations
    • ABNT:
      YOU, Q. et al. Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects. American Journal of Sports Medicine, [s. l.], v. 48, n. 3, p. 599–611, 2020. DOI 10.1177/0363546519897912. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=141996871&custid=s8280428. Acesso em: 28 out. 2020.
    • AMA:
      You Q, Liu Z, Zhang J, et al. Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects. American Journal of Sports Medicine. 2020;48(3):599-611. doi:10.1177/0363546519897912
    • APA:
      You, Q., Liu, Z., Zhang, J., Shen, M., Li, Y., Jin, Y., & Liu, Y. (2020). Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects. American Journal of Sports Medicine, 48(3), 599–611. https://doi.org/10.1177/0363546519897912
    • Chicago/Turabian: Author-Date:
      You, Qi, Ziming Liu, Jun Zhang, Mengjie Shen, Yuwan Li, Ying Jin, and Yi Liu. 2020. “Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects.” American Journal of Sports Medicine 48 (3): 599–611. doi:10.1177/0363546519897912.
    • Harvard:
      You, Q. et al. (2020) ‘Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects’, American Journal of Sports Medicine, 48(3), pp. 599–611. doi: 10.1177/0363546519897912.
    • Harvard: Australian:
      You, Q, Liu, Z, Zhang, J, Shen, M, Li, Y, Jin, Y & Liu, Y 2020, ‘Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects’, American Journal of Sports Medicine, vol. 48, no. 3, pp. 599–611, viewed 28 October 2020, .
    • MLA:
      You, Qi, et al. “Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects.” American Journal of Sports Medicine, vol. 48, no. 3, Mar. 2020, pp. 599–611. EBSCOhost, doi:10.1177/0363546519897912.
    • Chicago/Turabian: Humanities:
      You, Qi, Ziming Liu, Jun Zhang, Mengjie Shen, Yuwan Li, Ying Jin, and Yi Liu. “Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects.” American Journal of Sports Medicine 48, no. 3 (March 2020): 599–611. doi:10.1177/0363546519897912.
    • Vancouver/ICMJE:
      You Q, Liu Z, Zhang J, Shen M, Li Y, Jin Y, et al. Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects. American Journal of Sports Medicine [Internet]. 2020 Mar [cited 2020 Oct 28];48(3):599–611. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=141996871&custid=s8280428