Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Investigation on rat intestinal homeostasis alterations induced by 7-day simulated microgravity effect based on a proteomic approach.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • معلومة اضافية
    • Author-Supplied Keywords:
      HPLC-MS/MS
      Proteomics
      Rat intestine
      Simulated microgravity effect
    • NAICS/Industry Codes:
      112990 All Other Animal Production
    • Abstract:
      The present study aims at investigating alterations of rat intestinal homeostasis induced by 7-day simulated microgravity (SMG) effect through a proteomic approach. Tail-suspension model was used to simulate microgravity effect and a label-free quantitative proteomic strategy was employed to determine proteins in rat intestine. As a result, 717 differently expressed proteins were identified and 29 proteins were down-regulated while 688 proteins were up-regulated. 283 out of 717 proteins were categorized into 7 clusters with DAVID (version 6.8). The three highest enrichment scores were annotation cluster I about cell-cell adhesion (46 proteins with enrichment score of 16.67), annotation cluster II about carbohydrate metabolism (32 proteins with enrichment score of 6.17) and annotation cluster III about activity of pepdtidase (17 proteins with enrichment score of 4.74). Results of rat intestine proteomics indicate that SMG might disrupt intestinal homeostasis, which possibly resulted in opening of intestinal epithelial barrier (IEB), potentially leading to risk of systemic inflammatory response (SIR) and inflammatory bowel diseases (IBD). The present results also provide some useful information for mechanism and countermeasures of intestine injuries induced by microgravity. • A proteomic analysis of rat intestine under simulated microgravity was conducted based on label-free quantitative strategy. • 717 differentially expressed proteins was identified and categorized into 7 clusters. • Simulated microgravity might have influence on rat intestinal structural integrity, metabolism and oxidative stress. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Acta Astronautica is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1School of Life Science, Beijing Institute of Technology, No. 5 Zhongguangcun South Street, Haidian District, Beijing, 100081, China
      2Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 16 Nanxiaojie, Dongzhimen Nei Ave, Beijing, 100700, China
    • ISSN:
      0094-5765
    • Accession Number:
      10.1016/j.actaastro.2018.11.013
    • Accession Number:
      139978270
  • Citations
    • ABNT:
      WANG, S. et al. Investigation on rat intestinal homeostasis alterations induced by 7-day simulated microgravity effect based on a proteomic approach. Acta Astronautica, [s. l.], v. 166, p. 560–566, 2020. DOI 10.1016/j.actaastro.2018.11.013. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=139978270&custid=s8280428. Acesso em: 28 fev. 2020.
    • AMA:
      Wang S, Zhang Y, Guo J, Kang L, Deng Y, Li Y. Investigation on rat intestinal homeostasis alterations induced by 7-day simulated microgravity effect based on a proteomic approach. Acta Astronautica. 2020;166:560-566. doi:10.1016/j.actaastro.2018.11.013.
    • APA:
      Wang, S., Zhang, Y., Guo, J., Kang, L., Deng, Y., & Li, Y. (2020). Investigation on rat intestinal homeostasis alterations induced by 7-day simulated microgravity effect based on a proteomic approach. Acta Astronautica, 166, 560–566. https://doi.org/10.1016/j.actaastro.2018.11.013
    • Chicago/Turabian: Author-Date:
      Wang, Shibo, Yushi Zhang, Jingjing Guo, Liting Kang, Yulin Deng, and Yujuan Li. 2020. “Investigation on Rat Intestinal Homeostasis Alterations Induced by 7-Day Simulated Microgravity Effect Based on a Proteomic Approach.” Acta Astronautica 166 (January): 560–66. doi:10.1016/j.actaastro.2018.11.013.
    • Harvard:
      Wang, S. et al. (2020) ‘Investigation on rat intestinal homeostasis alterations induced by 7-day simulated microgravity effect based on a proteomic approach’, Acta Astronautica, 166, pp. 560–566. doi: 10.1016/j.actaastro.2018.11.013.
    • Harvard: Australian:
      Wang, S, Zhang, Y, Guo, J, Kang, L, Deng, Y & Li, Y 2020, ‘Investigation on rat intestinal homeostasis alterations induced by 7-day simulated microgravity effect based on a proteomic approach’, Acta Astronautica, vol. 166, pp. 560–566, viewed 28 February 2020, .
    • MLA:
      Wang, Shibo, et al. “Investigation on Rat Intestinal Homeostasis Alterations Induced by 7-Day Simulated Microgravity Effect Based on a Proteomic Approach.” Acta Astronautica, vol. 166, Jan. 2020, pp. 560–566. EBSCOhost, doi:10.1016/j.actaastro.2018.11.013.
    • Chicago/Turabian: Humanities:
      Wang, Shibo, Yushi Zhang, Jingjing Guo, Liting Kang, Yulin Deng, and Yujuan Li. “Investigation on Rat Intestinal Homeostasis Alterations Induced by 7-Day Simulated Microgravity Effect Based on a Proteomic Approach.” Acta Astronautica 166 (January 2020): 560–66. doi:10.1016/j.actaastro.2018.11.013.
    • Vancouver/ICMJE:
      Wang S, Zhang Y, Guo J, Kang L, Deng Y, Li Y. Investigation on rat intestinal homeostasis alterations induced by 7-day simulated microgravity effect based on a proteomic approach. Acta Astronautica [Internet]. 2020 Jan [cited 2020 Feb 28];166:560–6. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=139978270&custid=s8280428