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Comparison of the systemic phospholipid profile in dogs diagnosed with idiopathic inflammatory bowel disease or food-responsive diarrhea before and after treatment.

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  • معلومة اضافية
    • Author-Supplied Keywords:
      Amniotes
      Anatomy
      Animals
      Biochemistry
      Biology and life sciences
      Blood
      Blood plasma
      Body fluids
      Diet
      Dogs
      Eukaryota
      Gastroenterology and hepatology
      Inflammatory bowel disease
      Lipids
      Mammals
      Medicine and health sciences
      Nutrition
      Organisms
      Phospholipids
      Physiology
      Research and analysis methods
      Research Article
      Specimen preparation and treatment
      Vertebrates
      Veterinary diagnostics
      Veterinary medicine
      Veterinary science
    • NAICS/Industry Codes:
      414510 Pharmaceuticals and pharmacy supplies merchant wholesalers
      411110 Live animal merchant wholesalers
      112990 All Other Animal Production
    • Abstract:
      Background: Inflammatory bowel disease (IBD) and food-responsive diarrhea (FRD) are common chronic enteropathies in dogs, of which the exact pathogenesis has not been fully understood. In people dyslipidemia has been reported in patients with IBD, and potential therapeutic benefits of polyunsaturated fatty acids (PUFA) in the treatment of IBD have been investigated. Studies on the phospholipid profile in dogs with IBD and FRD are still lacking. Aim: To investigate the systemic phospholipid profile of dogs with IBD or FRD and to evaluate possible differences in phospholipids before and after treatment. Methods: The phospholipids in whole blood and EDTA plasma of 32 dogs diagnosed with either IBD (n = 16) or FRD (n = 16) were analyzed by hydrophilic interaction liquid chromatography (HILIC) prior to and after initiation of treatment, which included an elimination diet enriched with PUFAs. Results: A clear separation of the phospholipids between whole blood and plasma was demonstrated on principal component analysis plots. In addition to the type of specimen, treatment and disease severity were the most significant factors determining the variance of the phospholipid profile. An increase in lysolipids was observed after treatment. The phosphatidylcholine (PC) species changed from PC 38:4 before treatment to mainly lysophosphatidylcholine 18:0 after treatment. Furthermore, several differences in the abundance of individual phospholipids were identified between dogs with IBD and dogs with FRD and between treatment statuses using random forest analysis. Conclusion: Significant variances were identified in the phospholipid profiles of dogs with IBD and FRD. These were particularly determined by type of specimen used, disease severity and treatment status. After treatment, a shift of phospholipid species towards lysophosphatidylcholine 18:0 was observed. Future studies should further investigate the role of lipids in the pathophysiology of IBD and FRD as well as their potential therapeutic benefits. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • Author Affiliations:
      1Department for Small Animals, Veterinary Teaching Hospital, College of Veterinary Medicine, University of Leipzig, Leipzig, Saxony, Germany
      2Faculty of Veterinary Medicine, Department of Biochemistry & Cell Biology, Lipidomics Facility, Utrecht University, CM Utrecht, Netherlands
      3Division of Small Animal Internal Medicine, Department for Companion Animals and Horses, Vetmeduni Vienna, Vienna, Austria
    • ISSN:
      1932-6203
    • Accession Number:
      10.1371/journal.pone.0215435
    • Accession Number:
      135906996
  • Citations
    • ABNT:
      KALENYAK, K. et al. Comparison of the systemic phospholipid profile in dogs diagnosed with idiopathic inflammatory bowel disease or food-responsive diarrhea before and after treatment. PLoS ONE, [s. l.], v. 14, n. 4, p. 1–22, 2019. DOI 10.1371/journal.pone.0215435. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=135906996&custid=s8280428. Acesso em: 27 set. 2020.
    • AMA:
      Kalenyak K, Heilmann RM, van de Lest CHA, Brouwers JF, Burgener IA. Comparison of the systemic phospholipid profile in dogs diagnosed with idiopathic inflammatory bowel disease or food-responsive diarrhea before and after treatment. PLoS ONE. 2019;14(4):1-22. doi:10.1371/journal.pone.0215435
    • APA:
      Kalenyak, K., Heilmann, R. M., van de Lest, C. H. A., Brouwers, J. F., & Burgener, I. A. (2019). Comparison of the systemic phospholipid profile in dogs diagnosed with idiopathic inflammatory bowel disease or food-responsive diarrhea before and after treatment. PLoS ONE, 14(4), 1–22. https://doi.org/10.1371/journal.pone.0215435
    • Chicago/Turabian: Author-Date:
      Kalenyak, Katja, Romy M. Heilmann, Chris H. A. van de Lest, Jos F. Brouwers, and Iwan A. Burgener. 2019. “Comparison of the Systemic Phospholipid Profile in Dogs Diagnosed with Idiopathic Inflammatory Bowel Disease or Food-Responsive Diarrhea before and after Treatment.” PLoS ONE 14 (4): 1–22. doi:10.1371/journal.pone.0215435.
    • Harvard:
      Kalenyak, K. et al. (2019) ‘Comparison of the systemic phospholipid profile in dogs diagnosed with idiopathic inflammatory bowel disease or food-responsive diarrhea before and after treatment’, PLoS ONE, 14(4), pp. 1–22. doi: 10.1371/journal.pone.0215435.
    • Harvard: Australian:
      Kalenyak, K, Heilmann, RM, van de Lest, CHA, Brouwers, JF & Burgener, IA 2019, ‘Comparison of the systemic phospholipid profile in dogs diagnosed with idiopathic inflammatory bowel disease or food-responsive diarrhea before and after treatment’, PLoS ONE, vol. 14, no. 4, pp. 1–22, viewed 27 September 2020, .
    • MLA:
      Kalenyak, Katja, et al. “Comparison of the Systemic Phospholipid Profile in Dogs Diagnosed with Idiopathic Inflammatory Bowel Disease or Food-Responsive Diarrhea before and after Treatment.” PLoS ONE, vol. 14, no. 4, Apr. 2019, pp. 1–22. EBSCOhost, doi:10.1371/journal.pone.0215435.
    • Chicago/Turabian: Humanities:
      Kalenyak, Katja, Romy M. Heilmann, Chris H. A. van de Lest, Jos F. Brouwers, and Iwan A. Burgener. “Comparison of the Systemic Phospholipid Profile in Dogs Diagnosed with Idiopathic Inflammatory Bowel Disease or Food-Responsive Diarrhea before and after Treatment.” PLoS ONE 14, no. 4 (April 16, 2019): 1–22. doi:10.1371/journal.pone.0215435.
    • Vancouver/ICMJE:
      Kalenyak K, Heilmann RM, van de Lest CHA, Brouwers JF, Burgener IA. Comparison of the systemic phospholipid profile in dogs diagnosed with idiopathic inflammatory bowel disease or food-responsive diarrhea before and after treatment. PLoS ONE [Internet]. 2019 Apr 16 [cited 2020 Sep 27];14(4):1–22. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=135906996&custid=s8280428