Pre-activation of TLR3 enhances the therapeutic effect of BMMSCs through regulation the intestinal HIF-2α signaling pathway and balance of NKB cells in experimental alcoholic liver injury.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • معلومة اضافية
    • Author-Supplied Keywords:
      Alcoholic liver injury
      BMMSCs
      HIF-2α
      Intestinal barrier
      NKB cells
      TLR3
    • Abstract:
      Abstract Increased intestinal permeability and immune disorder are important mechanisms of alcoholic liver disease (ALD). Recent evidences suggest bone marrow derived mesenchymal stem cells (BMMSCs) have protective effects on end-stage liver disease and intestinal barrier injury. Moreover, the activation of toll-like receptor 3 (TLR3) has been shown enhancing therapeutic effects of BMMSCs in inflammatory bowel disease (IBD). However, the mechanism remains unclear. In current study, chronic-binge alcohol abuse model was employed to investigate the therapeutic effects of BMMSCs and BMMSCs pre-activated with TLR3 (P-BMMSCs) on alcohol-induced liver and intestine damage. C57BL/6 mice were divided into four groups with normal control, alcohol-fed model, alcohol-fed model with BMMSCs treatment and alcohol-fed model with P-BMMSCs treatment. Alcohol-fed mice were fed Lieber-DeCali diet containing 5% alcohol for four weeks and given alcohol intragastrically on the 28th day, but control group were fed isocaloric diet. BMMSCs and P-BMMSCs were injected into the treatment group three times. Results showed alcohol diet causing significant damage to intestinal barrier and liver. These were reversed by the treatment of BMMSCs, especially P-BMMSCs. Moreover, alcohol increased the expression of intestinal HIF-2α, the proportion of NKB cells and the level of serum IL-18, while BMMSCs or P-BMMSCs reduced these factors. In conclusion, BMMSCs, especially TLR3 pre-activated BMMSCs could be used to protect alcohol-induced intestine and liver injury. Highlights • BMMSCs especially TLR3 pre-activated BMMSCs ameliorate alcohol-induced liver and intestine injury in ALD mouse model. • BMMSCs especially TLR3 pre-activated BMMSCs reverse the expression of intestinal HIF-2α in ALD mouse model. • BMMSCs especially TLR3 pre-activated BMMSCs normalize NKB cells in spleen and the serum IL-18 level in ALD mouse model. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of International Immunopharmacology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Department of Infectious Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou Key Laboratory of Hepatology, Hepatology Institute of Wenzhou Medical University, Wenzhou 325000, China
    • Full Text Word Count:
      5265
    • ISSN:
      1567-5769
    • Accession Number:
      10.1016/j.intimp.2019.02.021
    • Accession Number:
      135600165
  • Citations
    • ABNT:
      GE, L. et al. Pre-activation of TLR3 enhances the therapeutic effect of BMMSCs through regulation the intestinal HIF-2α signaling pathway and balance of NKB cells in experimental alcoholic liver injury. International Immunopharmacology, [s. l.], v. 70, p. 477–485, 2019. DOI 10.1016/j.intimp.2019.02.021. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=135600165&custid=s8280428. Acesso em: 19 fev. 2020.
    • AMA:
      Ge L, Chen D, Chen W, et al. Pre-activation of TLR3 enhances the therapeutic effect of BMMSCs through regulation the intestinal HIF-2α signaling pathway and balance of NKB cells in experimental alcoholic liver injury. International Immunopharmacology. 2019;70:477-485. doi:10.1016/j.intimp.2019.02.021.
    • APA:
      Ge, L., Chen, D., Chen, W., Cai, C., Tao, Y., Ye, S., Lin, Z., Wang, X., Li, J., Xu, L., & Chen, Y. (2019). Pre-activation of TLR3 enhances the therapeutic effect of BMMSCs through regulation the intestinal HIF-2α signaling pathway and balance of NKB cells in experimental alcoholic liver injury. International Immunopharmacology, 70, 477–485. https://doi.org/10.1016/j.intimp.2019.02.021
    • Chicago/Turabian: Author-Date:
      Ge, Lichao, Dazhi Chen, Wenkai Chen, Chao Cai, Ying Tao, Shasha Ye, Zhuo Lin, et al. 2019. “Pre-Activation of TLR3 Enhances the Therapeutic Effect of BMMSCs through Regulation the Intestinal HIF-2α Signaling Pathway and Balance of NKB Cells in Experimental Alcoholic Liver Injury.” International Immunopharmacology 70 (May): 477–85. doi:10.1016/j.intimp.2019.02.021.
    • Harvard:
      Ge, L. et al. (2019) ‘Pre-activation of TLR3 enhances the therapeutic effect of BMMSCs through regulation the intestinal HIF-2α signaling pathway and balance of NKB cells in experimental alcoholic liver injury’, International Immunopharmacology, 70, pp. 477–485. doi: 10.1016/j.intimp.2019.02.021.
    • Harvard: Australian:
      Ge, L, Chen, D, Chen, W, Cai, C, Tao, Y, Ye, S, Lin, Z, Wang, X, Li, J, Xu, L & Chen, Y 2019, ‘Pre-activation of TLR3 enhances the therapeutic effect of BMMSCs through regulation the intestinal HIF-2α signaling pathway and balance of NKB cells in experimental alcoholic liver injury’, International Immunopharmacology, vol. 70, pp. 477–485, viewed 19 February 2020, .
    • MLA:
      Ge, Lichao, et al. “Pre-Activation of TLR3 Enhances the Therapeutic Effect of BMMSCs through Regulation the Intestinal HIF-2α Signaling Pathway and Balance of NKB Cells in Experimental Alcoholic Liver Injury.” International Immunopharmacology, vol. 70, May 2019, pp. 477–485. EBSCOhost, doi:10.1016/j.intimp.2019.02.021.
    • Chicago/Turabian: Humanities:
      Ge, Lichao, Dazhi Chen, Wenkai Chen, Chao Cai, Ying Tao, Shasha Ye, Zhuo Lin, et al. “Pre-Activation of TLR3 Enhances the Therapeutic Effect of BMMSCs through Regulation the Intestinal HIF-2α Signaling Pathway and Balance of NKB Cells in Experimental Alcoholic Liver Injury.” International Immunopharmacology 70 (May 2019): 477–85. doi:10.1016/j.intimp.2019.02.021.
    • Vancouver/ICMJE:
      Ge L, Chen D, Chen W, Cai C, Tao Y, Ye S, et al. Pre-activation of TLR3 enhances the therapeutic effect of BMMSCs through regulation the intestinal HIF-2α signaling pathway and balance of NKB cells in experimental alcoholic liver injury. International Immunopharmacology [Internet]. 2019 May [cited 2020 Feb 19];70:477–85. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=135600165&custid=s8280428