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Ultra-performance hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for simultaneous determination of allopurinol, oxypurinol and lesinurad in rat plasma: Application to pharmacokinetic study in rats.

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  • معلومة اضافية
    • Author-Supplied Keywords:
      Acetonitrile
      Acids
      Anatomy
      Biology and life sciences
      Blood
      Blood plasma
      Body fluids
      Carboxylic acids
      Chemical compounds
      Chemical reactions
      Chemistry
      Extraction techniques
      Formic acid
      Ionization
      Liquid-liquid extraction
      Medicine and health sciences
      Nitriles
      Oral administration
      Organic chemistry
      Organic compounds
      Pharmacokinetics
      Pharmacology
      Physical sciences
      Physiology
      Research and analysis methods
      Research Article
      Routes of administration
      Uric acid
    • NAICS/Industry Codes:
      112990 All Other Animal Production
    • Abstract:
      A fixed dose combination of lesinurad and allopurinol has been recently approved by USFDA and EMA for treatment of gout-associated hyperuricemia in patients who have not achieved target serum uric acid levels with allopurinol alone. In this study, an ultra-performance hydrophilic interaction liquid chromatography (UPHILIC) coupled with tandem mass spectrometry method was developed and validated for simultaneous determination of allopurinol, oxypurinol and lesinurad in rat plasma. Liquid liquid extraction using ethyl acetate as extracting agent was used for samples extraction procedure. Acquity UPLC HILIC column (100 mm x 2.1, 1.7μm) was used for separation of allopurinol, oxypurinol, lesinurad and internal standard (5-Florouracil). The mobile phase consisting of acetonitrile, water and formic acid (95:5:0.1, v/v/v), were eluted at 0.3 mL/min flow rate having total chromatographic run time of 3 min per sample. The analytes were detected on Acquity triple quadrupole mass spectrometer equipped with a Z-Spray electrospray ionization (ESI). The ESI source was operated in negative mode and multiple reaction monitoring was used for ion transition for all compounds. The precursor to product ion transition of m/z 134.94 > 64.07 for allopurinol, 150.89 > 41.91 for oxypurinol, 401.90 > 176.79 for lesinurad and 128.85 >41.92 for internal standard were used for identification and quantification. The calibration curves for all analytes were found to be linear with weighing factor of 1/x2 using regression analysis. The developed assay was successfully applied in an oral pharmacokinetic study of allopurinol, oxypurinol and lesinurad in rats. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
      2Bioavailability Laboratory, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
      3Medicinal Chemistry and Molecular Modelling Lab, Department of Pharmaceutical Chemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India
    • Full Text Word Count:
      5411
    • ISSN:
      1932-6203
    • Accession Number:
      10.1371/journal.pone.0213786
    • Accession Number:
      135310551
  • Citations
    • ABNT:
      IQBAL, M. et al. Ultra-performance hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for simultaneous determination of allopurinol, oxypurinol and lesinurad in rat plasma: Application to pharmacokinetic study in rats. PLoS ONE, [s. l.], v. 14, n. 3, p. 1–13, 2019. DOI 10.1371/journal.pone.0213786. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=135310551&custid=s8280428. Acesso em: 10 jul. 2020.
    • AMA:
      Iqbal M, Ezzeldin E, Herqash RN, Alam O. Ultra-performance hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for simultaneous determination of allopurinol, oxypurinol and lesinurad in rat plasma: Application to pharmacokinetic study in rats. PLoS ONE. 2019;14(3):1-13. doi:10.1371/journal.pone.0213786.
    • AMA11:
      Iqbal M, Ezzeldin E, Herqash RN, Alam O. Ultra-performance hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for simultaneous determination of allopurinol, oxypurinol and lesinurad in rat plasma: Application to pharmacokinetic study in rats. PLoS ONE. 2019;14(3):1-13. doi:10.1371/journal.pone.0213786
    • APA:
      Iqbal, M., Ezzeldin, E., Herqash, R. N., & Alam, O. (2019). Ultra-performance hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for simultaneous determination of allopurinol, oxypurinol and lesinurad in rat plasma: Application to pharmacokinetic study in rats. PLoS ONE, 14(3), 1–13. https://doi.org/10.1371/journal.pone.0213786
    • Chicago/Turabian: Author-Date:
      Iqbal, Muzaffar, Essam Ezzeldin, Rashed Naji Herqash, and Ozair Alam. 2019. “Ultra-Performance Hydrophilic Interaction Liquid Chromatography Coupled with Tandem Mass Spectrometry for Simultaneous Determination of Allopurinol, Oxypurinol and Lesinurad in Rat Plasma: Application to Pharmacokinetic Study in Rats.” PLoS ONE 14 (3): 1–13. doi:10.1371/journal.pone.0213786.
    • Harvard:
      Iqbal, M. et al. (2019) ‘Ultra-performance hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for simultaneous determination of allopurinol, oxypurinol and lesinurad in rat plasma: Application to pharmacokinetic study in rats’, PLoS ONE, 14(3), pp. 1–13. doi: 10.1371/journal.pone.0213786.
    • Harvard: Australian:
      Iqbal, M, Ezzeldin, E, Herqash, RN & Alam, O 2019, ‘Ultra-performance hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for simultaneous determination of allopurinol, oxypurinol and lesinurad in rat plasma: Application to pharmacokinetic study in rats’, PLoS ONE, vol. 14, no. 3, pp. 1–13, viewed 10 July 2020, .
    • MLA:
      Iqbal, Muzaffar, et al. “Ultra-Performance Hydrophilic Interaction Liquid Chromatography Coupled with Tandem Mass Spectrometry for Simultaneous Determination of Allopurinol, Oxypurinol and Lesinurad in Rat Plasma: Application to Pharmacokinetic Study in Rats.” PLoS ONE, vol. 14, no. 3, Mar. 2019, pp. 1–13. EBSCOhost, doi:10.1371/journal.pone.0213786.
    • Chicago/Turabian: Humanities:
      Iqbal, Muzaffar, Essam Ezzeldin, Rashed Naji Herqash, and Ozair Alam. “Ultra-Performance Hydrophilic Interaction Liquid Chromatography Coupled with Tandem Mass Spectrometry for Simultaneous Determination of Allopurinol, Oxypurinol and Lesinurad in Rat Plasma: Application to Pharmacokinetic Study in Rats.” PLoS ONE 14, no. 3 (March 14, 2019): 1–13. doi:10.1371/journal.pone.0213786.
    • Vancouver/ICMJE:
      Iqbal M, Ezzeldin E, Herqash RN, Alam O. Ultra-performance hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for simultaneous determination of allopurinol, oxypurinol and lesinurad in rat plasma: Application to pharmacokinetic study in rats. PLoS ONE [Internet]. 2019 Mar 14 [cited 2020 Jul 10];14(3):1–13. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=135310551&custid=s8280428