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Decreased GPIHBP1 protein levels in visceral adipose tissue partly underlie the hypertriglyceridemic phenotype in insulin resistance.

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  • معلومة اضافية
    • Author-Supplied Keywords:
      Adipose tissue
      Anatomy
      Animal cells
      Animal models
      Animal studies
      Biochemistry
      Biological tissue
      Biology and life sciences
      Blood
      Blood plasma
      Body fluids
      Cell biology
      Cellular types
      Chemical reactions
      Chemistry
      Diabetic endocrinology
      Endocrine physiology
      Endocrinology
      Endothelial cells
      Epithelial cells
      Epithelium
      Experimental organism systems
      Gene expression and vector techniques
      Hormones
      Hydrolysis
      Insulin
      Insulin resistance
      Lipolysis
      Medicine and health sciences
      Model organisms
      Molecular biology
      Molecular biology assays and analysis techniques
      Molecular biology techniques
      Mouse models
      Physical sciences
      Physiology
      Protein expression
      Research and analysis methods
      Research Article
    • Abstract:
      GPIHBP1 is a protein localized at the endothelial cell surface that facilitates triglyceride (TG) lipolysis by binding lipoprotein lipase (LPL). Whether Glycosyl Phosphatidyl Inositol high density lipoprotein binding protein 1 (GPIHBP1) function is impaired and may underlie the hyperTG phenotype observed in type 2 diabetes is not yet established. To elucidate the mechanism underlying impaired TG homeostasis in insulin resistance state we studied the effect of insulin on GPIHBP1 protein expression in human microvascular endothelial cells (HMVEC) under flow conditions. Next, we assessed visceral adipose tissue GPIHBP1 protein expression in type 2 diabetes Lepr db/db mouse model as well as in subjects with ranging levels of insulin resistance. We report that insulin reduces the expression of GPIHBP1 protein in HMVECs. Furthermore, GPIHBP1 protein expression in visceral adipose tissue in Lepr db/db mice is significantly reduced as is the active monomeric form of GPIHBP1 as compared to Leprdb/m mice. A similar decrease in GPIHBP1 protein was observed in subjects with increased body weight. GPIHBP1 protein expression was negatively associated with insulin and HOMA-IR. In conclusion, our data suggest that decreased GPIHBP1 availability in insulin resistant state may hamper peripheral lipolysis capacity. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • Author Affiliations:
      1Department of Experimental Vascular Medicine, Amsterdam University Medical Center, location AMC, Amsterdam, The Netherlands
      2Unidad de Gestión Clínica Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Complejo Hospitalario de Málaga (Virgen de la Victoria)/Universidad de Malaga, Malaga, Spain
      3CIBER Fisiopatologia de la Obesidad y Nutrición (CB06/03), Barcelona, Spain
      4Department of Vascular Medicine, Amsterdam University Medical Center, location AMC, Amsterdam, The Netherlands
    • Full Text Word Count:
      5902
    • ISSN:
      1932-6203
    • Accession Number:
      10.1371/journal.pone.0205858
    • Accession Number:
      132928379
  • Citations
    • ABNT:
      SURENDRAN, R. P. et al. Decreased GPIHBP1 protein levels in visceral adipose tissue partly underlie the hypertriglyceridemic phenotype in insulin resistance. PLoS ONE, [s. l.], v. 13, n. 11, p. 1–15, 2018. DOI 10.1371/journal.pone.0205858. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=132928379&custid=s8280428. Acesso em: 27 fev. 2020.
    • AMA:
      Surendran RP, Udayyapan SD, Clemente-Postigo M, et al. Decreased GPIHBP1 protein levels in visceral adipose tissue partly underlie the hypertriglyceridemic phenotype in insulin resistance. PLoS ONE. 2018;13(11):1-15. doi:10.1371/journal.pone.0205858.
    • APA:
      Surendran, R. P., Udayyapan, S. D., Clemente-Postigo, M., Havik, S. R., Schimmel, A. W. M., Tinahones, F., Nieuwdorp, M., & Dallinga-Thie, G. M. (2018). Decreased GPIHBP1 protein levels in visceral adipose tissue partly underlie the hypertriglyceridemic phenotype in insulin resistance. PLoS ONE, 13(11), 1–15. https://doi.org/10.1371/journal.pone.0205858
    • Chicago/Turabian: Author-Date:
      Surendran, R. Preethi, Shanti D. Udayyapan, Mercedes Clemente-Postigo, Stefan R. Havik, Alinda W. M. Schimmel, Fransisco Tinahones, Max Nieuwdorp, and Geesje M. Dallinga-Thie. 2018. “Decreased GPIHBP1 Protein Levels in Visceral Adipose Tissue Partly Underlie the Hypertriglyceridemic Phenotype in Insulin Resistance.” PLoS ONE 13 (11): 1–15. doi:10.1371/journal.pone.0205858.
    • Harvard:
      Surendran, R. P. et al. (2018) ‘Decreased GPIHBP1 protein levels in visceral adipose tissue partly underlie the hypertriglyceridemic phenotype in insulin resistance’, PLoS ONE, 13(11), pp. 1–15. doi: 10.1371/journal.pone.0205858.
    • Harvard: Australian:
      Surendran, RP, Udayyapan, SD, Clemente-Postigo, M, Havik, SR, Schimmel, AWM, Tinahones, F, Nieuwdorp, M & Dallinga-Thie, GM 2018, ‘Decreased GPIHBP1 protein levels in visceral adipose tissue partly underlie the hypertriglyceridemic phenotype in insulin resistance’, PLoS ONE, vol. 13, no. 11, pp. 1–15, viewed 27 February 2020, .
    • MLA:
      Surendran, R.Preethi, et al. “Decreased GPIHBP1 Protein Levels in Visceral Adipose Tissue Partly Underlie the Hypertriglyceridemic Phenotype in Insulin Resistance.” PLoS ONE, vol. 13, no. 11, Nov. 2018, pp. 1–15. EBSCOhost, doi:10.1371/journal.pone.0205858.
    • Chicago/Turabian: Humanities:
      Surendran, R. Preethi, Shanti D. Udayyapan, Mercedes Clemente-Postigo, Stefan R. Havik, Alinda W. M. Schimmel, Fransisco Tinahones, Max Nieuwdorp, and Geesje M. Dallinga-Thie. “Decreased GPIHBP1 Protein Levels in Visceral Adipose Tissue Partly Underlie the Hypertriglyceridemic Phenotype in Insulin Resistance.” PLoS ONE 13, no. 11 (November 9, 2018): 1–15. doi:10.1371/journal.pone.0205858.
    • Vancouver/ICMJE:
      Surendran RP, Udayyapan SD, Clemente-Postigo M, Havik SR, Schimmel AWM, Tinahones F, et al. Decreased GPIHBP1 protein levels in visceral adipose tissue partly underlie the hypertriglyceridemic phenotype in insulin resistance. PLoS ONE [Internet]. 2018 Nov 9 [cited 2020 Feb 27];13(11):1–15. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=132928379&custid=s8280428