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Metabolic profiling of femoral muscle from rats at different periods of time after death.

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  • معلومة اضافية
    • Author-Supplied Keywords:
      Analytical chemistry
      Anatomy
      Bioassays and physiological analysis
      Biochemistry
      Biology and life sciences
      Body temperature
      Chemistry
      Chromatographic techniques
      Liquid chromatography
      Liquid chromatography-mass spectrometry
      Mass spectrometry
      Mathematical and statistical techniques
      Mathematics
      Medicine and health sciences
      Metabolic analysis
      Metabolism
      Metabolites
      Metabolomics
      Multivariate analysis
      Muscles
      Musculoskeletal system
      Physical sciences
      Physiological parameters
      Physiology
      Principal component analysis
      Research and analysis methods
      Research Article
      Skeletal muscles
      Spectrum analysis techniques
      Statistical methods
      Statistics (mathematics)
    • NAICS/Industry Codes:
      112990 All Other Animal Production
    • Abstract:
      Clarification of postmortem metabolite changes can help characterize the process of biological degradation and facilitate investigations of forensic casework, especially in the estimation of postmortem interval (PMI). Metabolomics can provide information on the molecular profiles of tissues, which can aid in investigating postmortem metabolite changes. In this study, liquid chromatography-mass spectrometric (LC-MS) analysis was performed to identify the metabolic profiles of rat femoral muscle at ten periods of time after death within 168 h. The results obtained by reversed-phase liquid chromatography (RPLC)- and hydrophilic interaction liquid chromatography (HILIC)- electrospray ionization (ESI±) have revealed more than 16,000 features from all four datasets. Furthermore, 915 of these features were identified using an in-house database. Principal component analysis (PCA) demonstrated the time-specific features of molecular profiling at each period of time after death. Moreover, results from partial least squares projection to latent structures-discriminant analysis (PLS-DA) disclosed a strong association of metabolic alterations of at least 59 metabolites with the time since death, especially within 48 h after death, which expounds these metabolites as potential indicators in PMI estimation. Altogether, our results illustrate the potentiality of metabolic profiling in the evaluation of PMI and provide candidate metabolite markers with strong correlation with time since death for forensic purpose. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, China
    • ISSN:
      1932-6203
    • Accession Number:
      10.1371/journal.pone.0203920
    • Accession Number:
      131783034
  • Citations
    • ABNT:
      DU, T. et al. Metabolic profiling of femoral muscle from rats at different periods of time after death. PLoS ONE, [s. l.], v. 13, n. 9, p. 1–15, 2018. DOI 10.1371/journal.pone.0203920. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=131783034&custid=s8280428. Acesso em: 10 jul. 2020.
    • AMA:
      Du T, Lin Z, Xie Y, et al. Metabolic profiling of femoral muscle from rats at different periods of time after death. PLoS ONE. 2018;13(9):1-15. doi:10.1371/journal.pone.0203920.
    • AMA11:
      Du T, Lin Z, Xie Y, et al. Metabolic profiling of femoral muscle from rats at different periods of time after death. PLoS ONE. 2018;13(9):1-15. doi:10.1371/journal.pone.0203920
    • APA:
      Du, T., Lin, Z., Xie, Y., Ye, X., Tu, C., Jin, K., Xie, J., & Shen, Y. (2018). Metabolic profiling of femoral muscle from rats at different periods of time after death. PLoS ONE, 13(9), 1–15. https://doi.org/10.1371/journal.pone.0203920
    • Chicago/Turabian: Author-Date:
      Du, Tieshuai, Zebin Lin, Yaling Xie, Xing Ye, Chunyan Tu, Kaidi Jin, Jianhui Xie, and Yiwen Shen. 2018. “Metabolic Profiling of Femoral Muscle from Rats at Different Periods of Time after Death.” PLoS ONE 13 (9): 1–15. doi:10.1371/journal.pone.0203920.
    • Harvard:
      Du, T. et al. (2018) ‘Metabolic profiling of femoral muscle from rats at different periods of time after death’, PLoS ONE, 13(9), pp. 1–15. doi: 10.1371/journal.pone.0203920.
    • Harvard: Australian:
      Du, T, Lin, Z, Xie, Y, Ye, X, Tu, C, Jin, K, Xie, J & Shen, Y 2018, ‘Metabolic profiling of femoral muscle from rats at different periods of time after death’, PLoS ONE, vol. 13, no. 9, pp. 1–15, viewed 10 July 2020, .
    • MLA:
      Du, Tieshuai, et al. “Metabolic Profiling of Femoral Muscle from Rats at Different Periods of Time after Death.” PLoS ONE, vol. 13, no. 9, Sept. 2018, pp. 1–15. EBSCOhost, doi:10.1371/journal.pone.0203920.
    • Chicago/Turabian: Humanities:
      Du, Tieshuai, Zebin Lin, Yaling Xie, Xing Ye, Chunyan Tu, Kaidi Jin, Jianhui Xie, and Yiwen Shen. “Metabolic Profiling of Femoral Muscle from Rats at Different Periods of Time after Death.” PLoS ONE 13, no. 9 (September 14, 2018): 1–15. doi:10.1371/journal.pone.0203920.
    • Vancouver/ICMJE:
      Du T, Lin Z, Xie Y, Ye X, Tu C, Jin K, et al. Metabolic profiling of femoral muscle from rats at different periods of time after death. PLoS ONE [Internet]. 2018 Sep 14 [cited 2020 Jul 10];13(9):1–15. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=131783034&custid=s8280428