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Assessing the mechanistic target of rapamycin complex-1 pathway in response to resistance exercise and feeding in human skeletal muscle by multiplex assay.

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  • معلومة اضافية
    • Abstract:
      The mechanistic target of rapamycin complex-1 (mTORC-1) is a key nutrient and contraction-sensitive protein that regulates a pathway leading to skeletal muscle growth. Utilizing a multiplex assay, we aimed to examine the phosphorylation status of key mTORC-1-related signalling molecules in response to protein feeding and resistance exercise. Eight healthy men (age, 22.5 ± 3.1 years; mass, 80 ± 9 kg; 1-repetition maximum leg extension, 87 ± 5 kg) performed 4 sets of unilateral leg extensions until volitional failure. Immediately following the final set, all participants consumed a protein-enriched beverage. A single skeletal muscle biopsy was obtained from the vastus lateralis before (Pre) with further bilateral biopsies at 1 h (1 h exercised legs (FEDEX) and 1 h nonexercised legs (FED)) and 3 h (3 h FEDEX and 3 h FED) after drink ingestion. Phosphorylated AktSer473 was significantly elevated from Pre at 1 h FEDEX. Phosphorylated p70S6K1Thr412 was significantly increased above Pre at 1 h FEDEX and 1 h FED and was still significantly elevated at 3 h FEDEX but not 3 h FED. Phosphorylated rpS6Ser235/236 was also significantly increased above Pre at 1 h FEDEX and 1 h FED with 1 h FEDEX greater than 1 h FED. Our data highlight the utility of a multiplex assay to assess anabolic signalling molecules in response to protein feeding and resistance exercise in humans. Importantly, these changes are comparable with those as previously reported using standard immunoblotting and protein activity assays. [ABSTRACT FROM AUTHOR]
    • Abstract:
      La cible mécaniste du complexe 1 de la rapamycine (« mTORC-1 ») est un nutriment clé et une protéine sensible à la contraction qui régule une voie aboutissant à la croissance du muscle squelettique. En utilisant le dosage multiplex, on veut examiner le statut de phosphorylation de molécules clés de signalisation pertinentes de la mTORC-1 en réponse à l’alimentation protéique et à l’exercice contre résistance. Huit hommes en bonne santé (âge : 22,5 ± 3,1 ans, masse : 80 ± 9 kg, maximum sans répétition à l’extension du genou : 87 ± 5 kg) effectuent 4 séries d’extension unilatérale du genou jusqu’à l’incapacité volontaire. Immédiatement après la dernière série, les participants prennent une boisson protéinée. On prélève une seule biopsie du muscle vastus lateralis avant (« Pre ») puis d’autres biopsies bilatérales 1 h (1 h membre inférieur sollicité (« FEDEX ») et 1 h membre inférieur non sollicité (« FED »)) et 3 h après (3 h FEDEX et 3 h FED) avoir pris une boisson protéinée. On observe une augmentation significative de l’AktSer473 phosphorylé de Pre à 1 h FEDEX. On observe une augmentation significative de p70S6K1Thr412 phosphorylé supérieure à Pre à 1 h dans la condition FEDEX et 1 h dans la condition FED et la valeur demeure élevée à 3 h dans la condition FEDEX, mais pas à 3 h dans la condition FED. On observe aussi une augmentation significative de la rpS6Ser235/236 phosphorylée supérieure à Pre à 1 h dans la condition FEDEX et à 1 h dans la condition FED, mais l’augmentation est plus grande à 1 h dans la condition FEDEX. Nos données soulignent l’utilité du dosage multiplex pour l’évaluation des molécules de signalisation anabolique en réponse à l’alimentation protéique et à l’exercice contre résistance chez les humains. Fait à noter, ces variations sont comparables à celles rapportées antérieurement dans des recherches utilisant le buvardage et le dosage de l’activité protéique. [Traduit par la Rédaction] [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Applied Physiology, Nutrition & Metabolism is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Department of Kinesiology, McMaster University, Hamilton, ON L8S 4K1, Canada.
      2Department of Physiological Sciences, Federal University of Santa Catarina, Florianópolis, 88040-900, SC, Brazil.
    • ISSN:
      1715-5312
    • Accession Number:
      10.1139/apnm-2017-0852
    • Accession Number:
      131489044
  • Citations
    • ABNT:
      MCGLORY, C. et al. Assessing the mechanistic target of rapamycin complex-1 pathway in response to resistance exercise and feeding in human skeletal muscle by multiplex assay. Applied Physiology, Nutrition & Metabolism, [s. l.], v. 43, n. 9, p. 945–949, 2018. DOI 10.1139/apnm-2017-0852. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=131489044&custid=s8280428. Acesso em: 29 maio. 2020.
    • AMA:
      McGlory C, Nunes EA, Oikawa SY, Tsakiridis E, Phillips SM. Assessing the mechanistic target of rapamycin complex-1 pathway in response to resistance exercise and feeding in human skeletal muscle by multiplex assay. Applied Physiology, Nutrition & Metabolism. 2018;43(9):945-949. doi:10.1139/apnm-2017-0852.
    • APA:
      McGlory, C., Nunes, E. A., Oikawa, S. Y., Tsakiridis, E., & Phillips, S. M. (2018). Assessing the mechanistic target of rapamycin complex-1 pathway in response to resistance exercise and feeding in human skeletal muscle by multiplex assay. Applied Physiology, Nutrition & Metabolism, 43(9), 945–949. https://doi.org/10.1139/apnm-2017-0852
    • Chicago/Turabian: Author-Date:
      McGlory, Chris, Everson A. Nunes, Sara Y. Oikawa, Evangelia Tsakiridis, and Stuart M. Phillips. 2018. “Assessing the Mechanistic Target of Rapamycin Complex-1 Pathway in Response to Resistance Exercise and Feeding in Human Skeletal Muscle by Multiplex Assay.” Applied Physiology, Nutrition & Metabolism 43 (9): 945–49. doi:10.1139/apnm-2017-0852.
    • Harvard:
      McGlory, C. et al. (2018) ‘Assessing the mechanistic target of rapamycin complex-1 pathway in response to resistance exercise and feeding in human skeletal muscle by multiplex assay’, Applied Physiology, Nutrition & Metabolism, 43(9), pp. 945–949. doi: 10.1139/apnm-2017-0852.
    • Harvard: Australian:
      McGlory, C, Nunes, EA, Oikawa, SY, Tsakiridis, E & Phillips, SM 2018, ‘Assessing the mechanistic target of rapamycin complex-1 pathway in response to resistance exercise and feeding in human skeletal muscle by multiplex assay’, Applied Physiology, Nutrition & Metabolism, vol. 43, no. 9, pp. 945–949, viewed 29 May 2020, .
    • MLA:
      McGlory, Chris, et al. “Assessing the Mechanistic Target of Rapamycin Complex-1 Pathway in Response to Resistance Exercise and Feeding in Human Skeletal Muscle by Multiplex Assay.” Applied Physiology, Nutrition & Metabolism, vol. 43, no. 9, Sept. 2018, pp. 945–949. EBSCOhost, doi:10.1139/apnm-2017-0852.
    • Chicago/Turabian: Humanities:
      McGlory, Chris, Everson A. Nunes, Sara Y. Oikawa, Evangelia Tsakiridis, and Stuart M. Phillips. “Assessing the Mechanistic Target of Rapamycin Complex-1 Pathway in Response to Resistance Exercise and Feeding in Human Skeletal Muscle by Multiplex Assay.” Applied Physiology, Nutrition & Metabolism 43, no. 9 (September 2018): 945–49. doi:10.1139/apnm-2017-0852.
    • Vancouver/ICMJE:
      McGlory C, Nunes EA, Oikawa SY, Tsakiridis E, Phillips SM. Assessing the mechanistic target of rapamycin complex-1 pathway in response to resistance exercise and feeding in human skeletal muscle by multiplex assay. Applied Physiology, Nutrition & Metabolism [Internet]. 2018 Sep [cited 2020 May 29];43(9):945–9. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=131489044&custid=s8280428