Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

The murine female intestinal microbiota does not shift throughout the estrous cycle.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • معلومة اضافية
    • Author-Supplied Keywords:
      Anatomy
      Bacteria
      Biochemistry
      Biology and life sciences
      Computer and information sciences
      Data management
      Digestive system
      Estrogens
      Estrous cycle
      Gastrointestinal tract
      Genetics
      Genomics
      Hormones
      Maternal health
      Medical microbiology
      Medicine and health sciences
      Microbial genomics
      Microbial taxonomy
      Microbiology
      Microbiome
      Obstetrics and gynecology
      Organisms
      Physiological processes
      Physiology
      Pregnancy
      Research Article
      Sex hormones
      Taxonomy
      Women's health
    • Abstract:
      Pregnancy is accompanied by maternal physiological adaptations including metabolic, endocrine, immune, cardiovascular, skeletomuscular and neurological modifications that facilitate fetal and placental growth and development. Emerging evidence suggests that the maternal intestinal microbiota is modified over the course of healthy pregnancy. We have recently identified a maternal intestinal microbial shift within hours of conception; a shift that continued with advancing gestation. It is possible that maternal gut bacterial profiles might be associated with the known endocrine changes that accompany the female reproductive (estrous) cycle. Methods: To determine whether the estrous cycle influenced the shifts in the maternal intestinal microbiota, time-matched fecal pellets were collected daily for 3 consecutive estrous cycles from individually housed, non-pregnant female C57BL/6J mice (n = 10) fed a control diet. Estrous stage was identified by cell type predominance in vaginal cytological samples. The corresponding fecal pellets for each estrous stage were processed for bacterial 16S rRNA sequencing of the variable 3 (V3) region. Results: Estrous cycle stage accounted for a very small and not statistically significant proportion of the variation in the fecal microbiota according to PERMANOVA testing performed on Bray-Curtis dissimilarity scores. These values displayed no significant clustering of fecal microbial communities by estrous stage. Conclusion: The estrous cycle does not result in any significant shift in the intestinal microbial community in the reproductively mature, regularly cycling female mouse. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada
      2Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, Canada
      3Department of Medicine, McMaster University, Hamilton, Canada
      4Department of Obstetrics and Gynecology, McMaster University, Hamilton, Canada
      5Department of Pediatrics, McMaster University, Hamilton, Canada
    • ISSN:
      1932-6203
    • Accession Number:
      10.1371/journal.pone.0200729
    • Accession Number:
      130727432
  • Citations
    • ABNT:
      WALLACE, J. G. et al. The murine female intestinal microbiota does not shift throughout the estrous cycle. PLoS ONE, [s. l.], v. 13, n. 7, p. 1–12, 2018. DOI 10.1371/journal.pone.0200729. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=130727432&custid=s8280428. Acesso em: 10 ago. 2020.
    • AMA:
      Wallace JG, Potts RH, Szamosi JC, Surette MG, Sloboda DM. The murine female intestinal microbiota does not shift throughout the estrous cycle. PLoS ONE. 2018;13(7):1-12. doi:10.1371/journal.pone.0200729
    • APA:
      Wallace, J. G., Potts, R. H., Szamosi, J. C., Surette, M. G., & Sloboda, D. M. (2018). The murine female intestinal microbiota does not shift throughout the estrous cycle. PLoS ONE, 13(7), 1–12. https://doi.org/10.1371/journal.pone.0200729
    • Chicago/Turabian: Author-Date:
      Wallace, Jessica G., Ryan H. Potts, Jake C. Szamosi, Michael G. Surette, and Deborah M. Sloboda. 2018. “The Murine Female Intestinal Microbiota Does Not Shift throughout the Estrous Cycle.” PLoS ONE 13 (7): 1–12. doi:10.1371/journal.pone.0200729.
    • Harvard:
      Wallace, J. G. et al. (2018) ‘The murine female intestinal microbiota does not shift throughout the estrous cycle’, PLoS ONE, 13(7), pp. 1–12. doi: 10.1371/journal.pone.0200729.
    • Harvard: Australian:
      Wallace, JG, Potts, RH, Szamosi, JC, Surette, MG & Sloboda, DM 2018, ‘The murine female intestinal microbiota does not shift throughout the estrous cycle’, PLoS ONE, vol. 13, no. 7, pp. 1–12, viewed 10 August 2020, .
    • MLA:
      Wallace, Jessica G., et al. “The Murine Female Intestinal Microbiota Does Not Shift throughout the Estrous Cycle.” PLoS ONE, vol. 13, no. 7, July 2018, pp. 1–12. EBSCOhost, doi:10.1371/journal.pone.0200729.
    • Chicago/Turabian: Humanities:
      Wallace, Jessica G., Ryan H. Potts, Jake C. Szamosi, Michael G. Surette, and Deborah M. Sloboda. “The Murine Female Intestinal Microbiota Does Not Shift throughout the Estrous Cycle.” PLoS ONE 13, no. 7 (July 16, 2018): 1–12. doi:10.1371/journal.pone.0200729.
    • Vancouver/ICMJE:
      Wallace JG, Potts RH, Szamosi JC, Surette MG, Sloboda DM. The murine female intestinal microbiota does not shift throughout the estrous cycle. PLoS ONE [Internet]. 2018 Jul 16 [cited 2020 Aug 10];13(7):1–12. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=130727432&custid=s8280428