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In situ T regulatory cells and Th17 cytokines in paired samples of leprosy type 1 and type 2 reactions.

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  • معلومة اضافية
    • Author-Supplied Keywords:
      Animal cells
      Bacterial diseases
      Biology and life sciences
      Biopsy
      Blood cells
      Cell biology
      Cell enumeration techniques
      Cell-mediated immunity
      Cellular types
      Cytokines
      Developmental biology
      Diagnostic medicine
      Immune cells
      Immune physiology
      Immune system
      Immunity
      Immunology
      Immunostaining
      Infectious diseases
      Innate immune system
      Leprosy
      Lesions
      Medicine and health sciences
      Molecular development
      Neglected tropical diseases
      Pathology and laboratory medicine
      Physiology
      Regulatory T cells
      Research and analysis methods
      Research Article
      Signs and symptoms
      Specimen preparation and treatment
      Staining
      Surgical and invasive medical procedures
      T cells
      Tropical diseases
      White blood cells
    • Abstract:
      Leprosy is a complex chronic, infectious dermato-neurological disease that affects the skin and peripheral nerves especially during immuno-inflammatory episodes known as type 1/T1R and type 2/T2R reactions. This study investigated the in situ expression of CD25+Foxp3+ Treg cells and TGF-β1, IFN-γ, IL-17 in leprosy T1R and T2R. Tregs were evaluated in 114 skin biopsies from 74 leprosy patients: 56 T1R (28-paired reaction-free/reactional biopsies, 28 unpaired T1R), 18 T2R (12 paired reaction-free/reactional biopsies, 6 unpaired T2R). Double CD25+Foxp3+immunostained Treg cells obtained by automated platform (Ventana BenchMark XT, Roche, Mannheim, Germany) were counted (Nikon Eclipse E400 2mm2). Cytokine expression was evaluated by immunostaining in 96 biopsies (48 paired reaction-free/reactional lesions, 24 T1R, 24 T2R) using rabbit polyclonal anti human TGF-β1, IFN-γ, IL-17 antibodies (Santa Cruz Biotechnology CA, USA). Treg cell counts in leprosy reactional lesions were higher compared to reaction-free (p = 0.002). Treg numbers were higher in T1R compared to paired unreactional T1R lesions (p = 0.001). Similar frequency of Treg was seen in paired reactional versus unreactional T2R lesions. Higher expression of TGF-β, IFN-γ and IL-17 was seen in T2R lesions compared to T1R and reaction-free lesions. The increase in Treg cells during T1R suggests a suppressive role to control the exacerbated cellular immune response during T1R that can cause tissue and nerve damage. Evidences of upregulated Treg cells in TR1, which usually occurs in patients with Th1-Th17 immunity and the indications of the expression of Th17/IL-17 in T2R, which develops in patients with Th2-Treg profile, suggest plasticity of Treg-Th17 cells populations and a potential role for these cell populations in the immunopathogenesis of leprosy reactions. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • Author Affiliations:
      1Tropical Pathology and Public Health Institute, Federal University of Goiás, Goiânia, Brazil
      2Hospital A.C. Camargo, Centro Internacional de Pesquisa, São Paulo, São Paulo, Brazil
      3Rede D'Or Hospitals Network, Anatomic Pathology Department, São Paulo, Brazil
      4General Pathology, Faculty of Dentistry—University of São Paulo, São Paulo, Brazil
    • Full Text Word Count:
      5924
    • ISSN:
      1932-6203
    • Accession Number:
      10.1371/journal.pone.0196853
    • Accession Number:
      130058658
  • Citations
    • ABNT:
      COSTA, M. B. et al. In situ T regulatory cells and Th17 cytokines in paired samples of leprosy type 1 and type 2 reactions. PLoS ONE, [s. l.], v. 13, n. 6, p. 1–14, 2018. DOI 10.1371/journal.pone.0196853. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=130058658&custid=s8280428. Acesso em: 7 ago. 2020.
    • AMA:
      Costa MB, Hungria EM, Freitas AA, et al. In situ T regulatory cells and Th17 cytokines in paired samples of leprosy type 1 and type 2 reactions. PLoS ONE. 2018;13(6):1-14. doi:10.1371/journal.pone.0196853
    • APA:
      Costa, M. B., Hungria, E. M., Freitas, A. A., Sousa, A. L. O. M., Jampietro, J., Soares, F. A., & Stefani, M. M. A. (2018). In situ T regulatory cells and Th17 cytokines in paired samples of leprosy type 1 and type 2 reactions. PLoS ONE, 13(6), 1–14. https://doi.org/10.1371/journal.pone.0196853
    • Chicago/Turabian: Author-Date:
      Costa, Maurício Barcelos, Emerith Mayra. Hungria, Aline Araújo. Freitas, Ana Lúcia O. M. Sousa, Juliano Jampietro, Fernando A. Soares, and Mariane M. A. Stefani. 2018. “In Situ T Regulatory Cells and Th17 Cytokines in Paired Samples of Leprosy Type 1 and Type 2 Reactions.” PLoS ONE 13 (6): 1–14. doi:10.1371/journal.pone.0196853.
    • Harvard:
      Costa, M. B. et al. (2018) ‘In situ T regulatory cells and Th17 cytokines in paired samples of leprosy type 1 and type 2 reactions’, PLoS ONE, 13(6), pp. 1–14. doi: 10.1371/journal.pone.0196853.
    • Harvard: Australian:
      Costa, MB, Hungria, EM, Freitas, AA, Sousa, ALOM, Jampietro, J, Soares, FA & Stefani, MMA 2018, ‘In situ T regulatory cells and Th17 cytokines in paired samples of leprosy type 1 and type 2 reactions’, PLoS ONE, vol. 13, no. 6, pp. 1–14, viewed 7 August 2020, .
    • MLA:
      Costa, Maurício Barcelos, et al. “In Situ T Regulatory Cells and Th17 Cytokines in Paired Samples of Leprosy Type 1 and Type 2 Reactions.” PLoS ONE, vol. 13, no. 6, June 2018, pp. 1–14. EBSCOhost, doi:10.1371/journal.pone.0196853.
    • Chicago/Turabian: Humanities:
      Costa, Maurício Barcelos, Emerith Mayra. Hungria, Aline Araújo. Freitas, Ana Lúcia O. M. Sousa, Juliano Jampietro, Fernando A. Soares, and Mariane M. A. Stefani. “In Situ T Regulatory Cells and Th17 Cytokines in Paired Samples of Leprosy Type 1 and Type 2 Reactions.” PLoS ONE 13, no. 6 (June 8, 2018): 1–14. doi:10.1371/journal.pone.0196853.
    • Vancouver/ICMJE:
      Costa MB, Hungria EM, Freitas AA, Sousa ALOM, Jampietro J, Soares FA, et al. In situ T regulatory cells and Th17 cytokines in paired samples of leprosy type 1 and type 2 reactions. PLoS ONE [Internet]. 2018 Jun 8 [cited 2020 Aug 7];13(6):1–14. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=130058658&custid=s8280428