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A reverse-phase method revisited: Rapid high-yield preparation of giant unilamellar vesicles (GUVs) using emulsification followed by centrifugation.

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  • معلومة اضافية
    • Author-Supplied Keywords:
      Emulsion
      Encapsulation
      Giant liposome
      Giant vesicle
      Phase-contrast microscopy
      Reverse-phase method
    • Abstract:
      Giant vesicles (GVs) are commonly used as artificial membranes and microcompartments in biochemical and biophysical studies. The efficiency and/or yield of their production can be strongly affected by the conditions and procedures used, and thus many protocols have been developed. A reverse-phase protocol has been used to prepare liposomes for decades, and is reported to be suitable for preparing GVs. By revisiting a reverse-phase (W/O/W emulsification) mechanism, in this study we verified how simply and efficiently GVs could be generated using the following alternative method. First, phospholipid/diethyl ether was mixed with an aqueous solution containing sucrose to form an emulsion, and the mixture was centrifuged. The resulting GVs together with tubular and sheet aggregates in the lower water phases could be purified by a second centrifugation after the addition of a glucose aqueous solution. This “reverse-phase/centrifugation” method provided a large amount of GVs of ∼20 μm in diameter from egg phosphatidylcholine as well as multiple lipid components. Spherical GVs were obtained in the presence of physiological salt concentrations (phosphate-buffered saline, PBS) when phosphatidylglycerol was included. The appearance of microdomains on the vesicle membranes may indicate that giant unilamellar vesicles (GUVs) can form through this process. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Colloids & Surfaces A: Phys. Eng. Asp. is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Division of Chemistry for Materials, Graduate School of Engineering, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie, 514-8507, Japan
    • ISSN:
      0927-7757
    • Accession Number:
      10.1016/j.colsurfa.2018.02.060
    • Accession Number:
      128741147
  • Citations
    • ABNT:
      TSUMOTO, K. et al. A reverse-phase method revisited: Rapid high-yield preparation of giant unilamellar vesicles (GUVs) using emulsification followed by centrifugation. Colloids & Surfaces A: Phys. Eng. Asp, [s. l.], v. 546, p. 74–82, 2018. DOI 10.1016/j.colsurfa.2018.02.060. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=128741147&custid=s8280428. Acesso em: 10 jul. 2020.
    • AMA:
      Tsumoto K, Hayashi Y, Tabata J, Tomita M. A reverse-phase method revisited: Rapid high-yield preparation of giant unilamellar vesicles (GUVs) using emulsification followed by centrifugation. Colloids & Surfaces A: Phys Eng Asp. 2018;546:74-82. doi:10.1016/j.colsurfa.2018.02.060.
    • AMA11:
      Tsumoto K, Hayashi Y, Tabata J, Tomita M. A reverse-phase method revisited: Rapid high-yield preparation of giant unilamellar vesicles (GUVs) using emulsification followed by centrifugation. Colloids & Surfaces A: Phys Eng Asp. 2018;546:74-82. doi:10.1016/j.colsurfa.2018.02.060
    • APA:
      Tsumoto, K., Hayashi, Y., Tabata, J., & Tomita, M. (2018). A reverse-phase method revisited: Rapid high-yield preparation of giant unilamellar vesicles (GUVs) using emulsification followed by centrifugation. Colloids & Surfaces A: Phys. Eng. Asp, 546, 74–82. https://doi.org/10.1016/j.colsurfa.2018.02.060
    • Chicago/Turabian: Author-Date:
      Tsumoto, Kanta, Yuki Hayashi, Jin Tabata, and Masahiro Tomita. 2018. “A Reverse-Phase Method Revisited: Rapid High-Yield Preparation of Giant Unilamellar Vesicles (GUVs) Using Emulsification Followed by Centrifugation.” Colloids & Surfaces A: Phys. Eng. Asp 546 (June): 74–82. doi:10.1016/j.colsurfa.2018.02.060.
    • Harvard:
      Tsumoto, K. et al. (2018) ‘A reverse-phase method revisited: Rapid high-yield preparation of giant unilamellar vesicles (GUVs) using emulsification followed by centrifugation’, Colloids & Surfaces A: Phys. Eng. Asp, 546, pp. 74–82. doi: 10.1016/j.colsurfa.2018.02.060.
    • Harvard: Australian:
      Tsumoto, K, Hayashi, Y, Tabata, J & Tomita, M 2018, ‘A reverse-phase method revisited: Rapid high-yield preparation of giant unilamellar vesicles (GUVs) using emulsification followed by centrifugation’, Colloids & Surfaces A: Phys. Eng. Asp, vol. 546, pp. 74–82, viewed 10 July 2020, .
    • MLA:
      Tsumoto, Kanta, et al. “A Reverse-Phase Method Revisited: Rapid High-Yield Preparation of Giant Unilamellar Vesicles (GUVs) Using Emulsification Followed by Centrifugation.” Colloids & Surfaces A: Phys. Eng. Asp, vol. 546, June 2018, pp. 74–82. EBSCOhost, doi:10.1016/j.colsurfa.2018.02.060.
    • Chicago/Turabian: Humanities:
      Tsumoto, Kanta, Yuki Hayashi, Jin Tabata, and Masahiro Tomita. “A Reverse-Phase Method Revisited: Rapid High-Yield Preparation of Giant Unilamellar Vesicles (GUVs) Using Emulsification Followed by Centrifugation.” Colloids & Surfaces A: Phys. Eng. Asp 546 (June 5, 2018): 74–82. doi:10.1016/j.colsurfa.2018.02.060.
    • Vancouver/ICMJE:
      Tsumoto K, Hayashi Y, Tabata J, Tomita M. A reverse-phase method revisited: Rapid high-yield preparation of giant unilamellar vesicles (GUVs) using emulsification followed by centrifugation. Colloids & Surfaces A: Phys Eng Asp [Internet]. 2018 Jun 5 [cited 2020 Jul 10];546:74–82. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=128741147&custid=s8280428