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Mansonella perstans microfilaremic individuals are characterized by enhanced type 2 helper T and regulatory T and B cell subsets and dampened systemic innate and adaptive immune responses.

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  • معلومة اضافية
    • Author-Supplied Keywords:
      Anatomy
      Animal cells
      Animals
      Antibodies
      Biochemistry
      Biology and life sciences
      Blood
      Blood cells
      Body fluids
      Cell biology
      Cellular types
      Cytokines
      Developmental biology
      Eukaryota
      Helminths
      Immune cells
      Immune physiology
      Immune response
      Immune system
      Immune system proteins
      Immunology
      Innate immune system
      Invertebrates
      Medicine and health sciences
      Molecular development
      Nematoda
      Onchocerca
      Onchocerca volvulus
      Organisms
      Physiology
      Proteins
      Regulatory T cells
      Research Article
      T cells
      White blood cells
    • Abstract:
      The filarial nematode Mansonella perstans is endemic throughout Africa, northern South America and the Caribbean. Interestingly, M. perstans-infected individuals present no distinct clinical picture associated with certain pathology. Due to its relatively silent nature, research on this tropical disease has been neglected, especially M. perstans-driven immune responses. A hindrance in obtaining data on M. perstans-specific responses has been the inability to obtain adult worms since their habitats in serous cavities are difficult to access. Thus, in this study, for the first time, we used Mansonella perstans worm antigen extract as stimulant to obtain filarial-specific recall and immunoglobulin responses from M. perstans microfilaremic individuals (Mp MF+) from Cameroon. Moreover, systemic immune profiles in sera and immune cell composition in peripheral blood from Mp MF+ and amicrofilaremic individuals (Mp MF-) were obtained. Our data reveal that Mp MF+ individuals showed significantly reduced cytokine (IL-4, IL-6 and IL-12p70) and chemokine levels (IL-8 and RANTES), but significantly higher MIP-1β as well as increased M. perstans-specific IgG4 levels compared to Mp MF- individuals. In contrast, upon re-stimulation with worm antigen extract, IFN-γ, IL-13, IL-10 and IL-17A secretion was enhanced in cell cultures from Mp MF+ individuals when compared to those from cultures of healthy European individuals. Moreover, analysis of immune cell composition in peripheral blood from Mp MF+ individuals revealed increased type 2 helper T (Th2), natural killer (NK), regulatory B and T cell (Breg and Treg) subsets but decreased type 1 regulatory T (Tr1) cells. In summary, this study deciphers for the first time, M. perstans-specific immune responses using worm antigen extract and shows that patent M. perstans infections have distinct Th2, Breg and Treg subsets accompanied with reduced systemic innate and adaptive immune responses and dominant filarial-specific IgG4 levels. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • Author Affiliations:
      1Institute of Medical Microbiology, Immunology and Parasitology, University Hospital of Bonn, Germany
      2Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon
      3Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon
      4Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children’s Hospital, Medical Faculty, Duesseldorf, Germany
      5German Centre for Infection Research (DZIF), partner site, Bonn-Cologne, Bonn, Germany
    • Full Text Word Count:
      11040
    • ISSN:
      1935-2727
    • Accession Number:
      10.1371/journal.pntd.0006184
    • Accession Number:
      127246046
  • Citations
    • ABNT:
      RITTER, M. et al. Mansonella perstans microfilaremic individuals are characterized by enhanced type 2 helper T and regulatory T and B cell subsets and dampened systemic innate and adaptive immune responses. PLoS Neglected Tropical Diseases, [s. l.], v. 12, n. 1, p. 1–21, 2018. DOI 10.1371/journal.pntd.0006184. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=127246046&custid=s8280428. Acesso em: 15 ago. 2020.
    • AMA:
      Ritter M, Ndongmo WPC, Njouendou AJ, et al. Mansonella perstans microfilaremic individuals are characterized by enhanced type 2 helper T and regulatory T and B cell subsets and dampened systemic innate and adaptive immune responses. PLoS Neglected Tropical Diseases. 2018;12(1):1-21. doi:10.1371/journal.pntd.0006184
    • APA:
      Ritter, M., Ndongmo, W. P. C., Njouendou, A. J., Nghochuzie, N. N., Nchang, L. C., Tayong, D. B., Arndts, K., Nausch, N., Jacobsen, M., Wanji, S., Layland, L. E., & Hoerauf, A. (2018). Mansonella perstans microfilaremic individuals are characterized by enhanced type 2 helper T and regulatory T and B cell subsets and dampened systemic innate and adaptive immune responses. PLoS Neglected Tropical Diseases, 12(1), 1–21. https://doi.org/10.1371/journal.pntd.0006184
    • Chicago/Turabian: Author-Date:
      Ritter, Manuel, Winston Patrick Chounna Ndongmo, Abdel Jelil Njouendou, Nora Nganyewo Nghochuzie, Lucy Cho Nchang, Dizzle Bita Tayong, Kathrin Arndts, et al. 2018. “Mansonella Perstans Microfilaremic Individuals Are Characterized by Enhanced Type 2 Helper T and Regulatory T and B Cell Subsets and Dampened Systemic Innate and Adaptive Immune Responses.” PLoS Neglected Tropical Diseases 12 (1): 1–21. doi:10.1371/journal.pntd.0006184.
    • Harvard:
      Ritter, M. et al. (2018) ‘Mansonella perstans microfilaremic individuals are characterized by enhanced type 2 helper T and regulatory T and B cell subsets and dampened systemic innate and adaptive immune responses’, PLoS Neglected Tropical Diseases, 12(1), pp. 1–21. doi: 10.1371/journal.pntd.0006184.
    • Harvard: Australian:
      Ritter, M, Ndongmo, WPC, Njouendou, AJ, Nghochuzie, NN, Nchang, LC, Tayong, DB, Arndts, K, Nausch, N, Jacobsen, M, Wanji, S, Layland, LE & Hoerauf, A 2018, ‘Mansonella perstans microfilaremic individuals are characterized by enhanced type 2 helper T and regulatory T and B cell subsets and dampened systemic innate and adaptive immune responses’, PLoS Neglected Tropical Diseases, vol. 12, no. 1, pp. 1–21, viewed 15 August 2020, .
    • MLA:
      Ritter, Manuel, et al. “Mansonella Perstans Microfilaremic Individuals Are Characterized by Enhanced Type 2 Helper T and Regulatory T and B Cell Subsets and Dampened Systemic Innate and Adaptive Immune Responses.” PLoS Neglected Tropical Diseases, vol. 12, no. 1, Jan. 2018, pp. 1–21. EBSCOhost, doi:10.1371/journal.pntd.0006184.
    • Chicago/Turabian: Humanities:
      Ritter, Manuel, Winston Patrick Chounna Ndongmo, Abdel Jelil Njouendou, Nora Nganyewo Nghochuzie, Lucy Cho Nchang, Dizzle Bita Tayong, Kathrin Arndts, et al. “Mansonella Perstans Microfilaremic Individuals Are Characterized by Enhanced Type 2 Helper T and Regulatory T and B Cell Subsets and Dampened Systemic Innate and Adaptive Immune Responses.” PLoS Neglected Tropical Diseases 12, no. 1 (January 11, 2018): 1–21. doi:10.1371/journal.pntd.0006184.
    • Vancouver/ICMJE:
      Ritter M, Ndongmo WPC, Njouendou AJ, Nghochuzie NN, Nchang LC, Tayong DB, et al. Mansonella perstans microfilaremic individuals are characterized by enhanced type 2 helper T and regulatory T and B cell subsets and dampened systemic innate and adaptive immune responses. PLoS Neglected Tropical Diseases [Internet]. 2018 Jan 11 [cited 2020 Aug 15];12(1):1–21. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=127246046&custid=s8280428