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Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring.

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  • معلومة اضافية
    • Author-Supplied Keywords:
      Anatomy
      Arthritis
      Articular cartilage
      Biochemistry
      Biological tissue
      Biology and life sciences
      Cartilage
      Collagens
      Connective tissue
      Diagnostic medicine
      Diagnostic radiology
      Gene expression
      Genetics
      Histology
      Imaging techniques
      Joints (anatomy)
      Knee joints
      Magnetic resonance imaging
      Medicine and health sciences
      Musculoskeletal system
      Osteoarthritis
      Proteins
      Radiology and imaging
      Research and analysis methods
      Research Article
      Rheumatology
    • NAICS/Industry Codes:
      621512 Diagnostic Imaging Centers
    • Abstract:
      Introduction: The Göttingen Minipig (GM) is used as large animal model in articular cartilage research. The aim of the study was to introduce osteoarthritis (OA) in the GM by resecting the anterior cruciate ligament (ACLR) according to Pond and Nuki, verified by histological and magnetic resonance imaging (MRI) scoring as well as analysis of gene and protein expression. Materials and Methods: The eight included skeletally mature female GM were assessed after ACLR in the left and a sham operation in the right knee, which served as control. 26 weeks after surgery the knee joints were scanned using a 3-Tesla high-field MR tomography unit with a 3 T CP Large Flex Coil. Standard proton-density weighted fat saturated sequences in coronal and sagittal direction with a slice thickness of 3 mm were used. The MRI scans were assessed by two radiologists according to a modified WORMS-score, the X-rays of the knee joints by two evaluators. Osteochondral plugs with a diameter of 4mm were taken for histological examination from either the main loading zone or the macroscopic most degenerated parts of the tibia plateau or condyle respectively. The histological sections were blinded and scored by three experts according to Little et al. Gene expression analysis was performed from surrounding cartilage. Expression of adamts4, adamts5, acan, col1A1, col2, il-1ß, mmp1, mmp3, mmp13, vegf was determined by qRT-PCR. Immunohistochemical staining (IH) of Col I and II was performed. IH was scored using a 4 point grading (0—no staining; 3-intense staining). Results and Discussion: Similar signs of OA were evident both in ACLR and sham operated knee joints with the histological scoring result of the ACLR joints with 6.48 ± 5.67 points and the sham joints with 6.86 ± 5.84 points (p = 0.7953) The MRI scoring yielded 0.34 ± 0.89 points for the ACLR and 0.03 ± 0.17 for the sham knee joints. There was no correlation between the histological and MRI scores (r = 0.10021). The gene expression profiles as well as the immunohistochemical findings showed no significant differences between ACLR and sham knee joints. In conclusion, both knee joints showed histological signs of OA after 26 weeks irrespective of whether the ACL was resected or not. As MRI results did not match the histological findings, MRI was obviously unsuitable to diagnose the OA in GM. The analysis of the expression patterns of the 10 genes could not shed light on the question, whether sham operation also induced cartilage erosion or if the degeneration was spontaneous. The modified Pond-Nuki model may be used with reservation in the adult minipig to induce an isolated osteoarthritis. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • Author Affiliations:
      1Department for experimental Orthopaedics and Trauma Surgery, Orthopaedic and Trauma Surgery Centre (OUZ), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany
      2Research Centre for Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Heidelberg, Germany
      3Institute of Clinical Radiology and Nuclear Medicine, University Medical Center Mannheim, Mannheim, Germany
    • ISSN:
      1932-6203
    • Accession Number:
      10.1371/journal.pone.0165897
    • Accession Number:
      119303313
  • Citations
    • ABNT:
      REISIG, G. et al. Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring. PLoS ONE, [s. l.], v. 11, n. 11, p. 1–27, 2016. DOI 10.1371/journal.pone.0165897. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=119303313&custid=s8280428. Acesso em: 25 maio. 2020.
    • AMA:
      Reisig G, Kreinest M, Richter W, et al. Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring. PLoS ONE. 2016;11(11):1-27. doi:10.1371/journal.pone.0165897.
    • APA:
      Reisig, G., Kreinest, M., Richter, W., Wagner-Ecker, M., Dinter, D., Attenberger, U., Schneider-Wald, B., Fickert, S., & Schwarz, M. L. (2016). Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring. PLoS ONE, 11(11), 1–27. https://doi.org/10.1371/journal.pone.0165897
    • Chicago/Turabian: Author-Date:
      Reisig, Gregor, Michael Kreinest, Wiltrud Richter, Mechthild Wagner-Ecker, Dietmar Dinter, Ulrike Attenberger, Barbara Schneider-Wald, Stefan Fickert, and Markus L. Schwarz. 2016. “Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring.” PLoS ONE 11 (11): 1–27. doi:10.1371/journal.pone.0165897.
    • Harvard:
      Reisig, G. et al. (2016) ‘Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring’, PLoS ONE, 11(11), pp. 1–27. doi: 10.1371/journal.pone.0165897.
    • Harvard: Australian:
      Reisig, G, Kreinest, M, Richter, W, Wagner-Ecker, M, Dinter, D, Attenberger, U, Schneider-Wald, B, Fickert, S & Schwarz, ML 2016, ‘Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring’, PLoS ONE, vol. 11, no. 11, pp. 1–27, viewed 25 May 2020, .
    • MLA:
      Reisig, Gregor, et al. “Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring.” PLoS ONE, vol. 11, no. 11, Nov. 2016, pp. 1–27. EBSCOhost, doi:10.1371/journal.pone.0165897.
    • Chicago/Turabian: Humanities:
      Reisig, Gregor, Michael Kreinest, Wiltrud Richter, Mechthild Wagner-Ecker, Dietmar Dinter, Ulrike Attenberger, Barbara Schneider-Wald, Stefan Fickert, and Markus L. Schwarz. “Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring.” PLoS ONE 11, no. 11 (November 7, 2016): 1–27. doi:10.1371/journal.pone.0165897.
    • Vancouver/ICMJE:
      Reisig G, Kreinest M, Richter W, Wagner-Ecker M, Dinter D, Attenberger U, et al. Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring. PLoS ONE [Internet]. 2016 Nov 7 [cited 2020 May 25];11(11):1–27. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=119303313&custid=s8280428