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Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise.

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  • معلومة اضافية
    • NAICS/Industry Codes:
      325410 Pharmaceutical and medicine manufacturing
      325411 Medicinal and Botanical Manufacturing
    • Abstract:
      Current evidence on exercise-mediated AMPK regulation in skeletal muscle of patients with type 2 diabetes (T2D) is inconclusive. This may relate to inadequate segregation of trimeric complexes in the investigation of AMPK activity. We examined the regulation of AMPK and downstream targets ACC-β, TBC1D1, and TBC1D4 in muscle biopsy specimens obtained from 13 overweight/obese patients with T2D and 14 weight-matched male control subjects before, immediately after, and 3 h after exercise. Exercise increased AMPK α2β2γ3 activity and phosphorylation of ACCβ Ser(221), TBC1D1 Ser(237)/Thr(596), and TBC1D4 Ser(704) Conversely, exercise decreased AMPK α1β2γ1 activity and TBC1D4 Ser(318)/Thr(642) phosphorylation. Interestingly, compared with preexercise, 3 h into exercise recovery, AMPK α2β2γ1 and α1β2γ1 activity were increased concomitant with increased TBC1D4 Ser(318)/Ser(341)/Ser(704) phosphorylation. No differences in these responses were observed between patients with T2D and control subjects. Subjects were also studied by euglycemic-hyperinsulinemic clamps performed at rest and 3 h after exercise. We found no evidence for insulin to regulate AMPK activity. Thus, AMPK signaling is not compromised in muscle of patients with T2D during exercise and insulin stimulation. Our results reveal a hitherto unrecognized activation of specific AMPK complexes in exercise recovery. We hypothesize that the differential regulation of AMPK complexes plays an important role for muscle metabolism and adaptations to exercise. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Diabetes is the property of American Diabetes Association and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Section of Molecular Physiology, August Krogh Centre, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Copenhagen, Denmark
      2Department of Endocrinology, Odense University Hospital, Odense, Denmark
      3Section of Molecular Diabetes and Metabolism, Institute of Molecular Medicine and Institute of Clinical Research, University of Southern Denmark, Odense, Denmark
    • ISSN:
      0012-1797
    • Accession Number:
      10.2337/db15-1034
    • Accession Number:
      114741057
  • Citations
    • ABNT:
      KJØBSTED, R. et al. Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise. Diabetes, [s. l.], v. 65, n. 5, p. 1219–1230, 2016. DOI 10.2337/db15-1034. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=114741057&custid=s8280428. Acesso em: 11 ago. 2020.
    • AMA:
      Kjøbsted R, Pedersen AJT, Hingst JR, et al. Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise. Diabetes. 2016;65(5):1219-1230. doi:10.2337/db15-1034
    • APA:
      Kjøbsted, R., Pedersen, A. J. T., Hingst, J. R., Sabaratnam, R., Birk, J. B., Kristensen, J. M., Højlund, K., & Wojtaszewski, J. F. P. (2016). Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise. Diabetes, 65(5), 1219–1230. https://doi.org/10.2337/db15-1034
    • Chicago/Turabian: Author-Date:
      Kjøbsted, Rasmus, Andreas J.T. Pedersen, Janne R. Hingst, Rugivan Sabaratnam, Jesper B. Birk, Jonas M. Kristensen, Kurt Højlund, and Jørgen F.P. Wojtaszewski. 2016. “Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise.” Diabetes 65 (5): 1219–30. doi:10.2337/db15-1034.
    • Harvard:
      Kjøbsted, R. et al. (2016) ‘Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise’, Diabetes, 65(5), pp. 1219–1230. doi: 10.2337/db15-1034.
    • Harvard: Australian:
      Kjøbsted, R, Pedersen, AJT, Hingst, JR, Sabaratnam, R, Birk, JB, Kristensen, JM, Højlund, K & Wojtaszewski, JFP 2016, ‘Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise’, Diabetes, vol. 65, no. 5, pp. 1219–1230, viewed 11 August 2020, .
    • MLA:
      Kjøbsted, Rasmus, et al. “Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise.” Diabetes, vol. 65, no. 5, May 2016, pp. 1219–1230. EBSCOhost, doi:10.2337/db15-1034.
    • Chicago/Turabian: Humanities:
      Kjøbsted, Rasmus, Andreas J.T. Pedersen, Janne R. Hingst, Rugivan Sabaratnam, Jesper B. Birk, Jonas M. Kristensen, Kurt Højlund, and Jørgen F.P. Wojtaszewski. “Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise.” Diabetes 65, no. 5 (May 2016): 1219–30. doi:10.2337/db15-1034.
    • Vancouver/ICMJE:
      Kjøbsted R, Pedersen AJT, Hingst JR, Sabaratnam R, Birk JB, Kristensen JM, et al. Intact Regulation of the AMPK Signaling Network in Response to Exercise and Insulin in Skeletal Muscle of Male Patients With Type 2 Diabetes: Illumination of AMPK Activation in Recovery From Exercise. Diabetes [Internet]. 2016 May [cited 2020 Aug 11];65(5):1219–30. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=114741057&custid=s8280428