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Influence of ageing and essential amino acids on quantitative patterns of troponin T alternative splicing in human skeletal muscle.

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  • معلومة اضافية
    • Author-Supplied Keywords:
      age
      amino acids
      mTOR
      muscle
      resistance exercise
      troponin T
      âge
      acides aminés
      exercice contre résistance
      mTOR
      muscle
      troponine T
      Language of Keywords: English; French
    • NAICS/Industry Codes:
      414510 Pharmaceuticals and pharmacy supplies merchant wholesalers
      446191 Food (Health) Supplement Stores
      111421 Nursery and Tree Production
    • Abstract:
      Ageing is associated with a loss of skeletal muscle performance, a condition referred to as sarcopenia. In part, the age-related reduction in performance is due to a selective loss of muscle fiber mass, but mass-independent effects have also been demonstrated. An important mass-independent determinant of muscle performance is the pattern of expression of isoforms of proteins that participate in muscle contraction (e.g., the troponins). In the present study, we tested the hypothesis that ageing impairs alternative splicing of the pre-mRNA encoding fast skeletal muscle troponin T ( TNNT3) in human vastus lateralis muscle. Furthermore, we hypothesized that resistance exercise alone or in combination with consumption of essential amino acids would attenuate age-associated effects on TNNT3 alternative splicing. Our results indicate that ageing negatively affects the pattern of TNNT3 alternative splicing in a manner that correlates quantitatively with age-associated reductions in muscle performance. Interestingly, whereas vastus lateralis TNNT3 alternative splicing was unaffected by a bout of resistance exercise 24 h prior to muscle biopsy, ingestion of a mixture of essential amino acids after resistance exercise resulted in a significant shift in the pattern of TNNT3 splice form expression in both age groups to one predicted to promote greater muscle performance. We conclude that essential amino acid supplementation after resistance exercise may provide a means to reduce impairments in skeletal muscle quality during ageing in humans. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Le vieillissement est associé à la diminution de la performance musculosquelettique, une condition associée à la sarcopénie. La baisse de performance liée à l'âge est due en partie à la diminution sélective de la masse des fibres musculaires; toutefois, des études révèlent une baisse de performance indépendante de la masse musculaire. Parmi les facteurs de la performance musculaire indépendants de la masse, il y a le mode d'expression des isoformes des protéines impliquées dans la contraction musculaire, la troponine par exemple. Dans la présente étude, on vérifie l'hypothèse selon laquelle le vieillissement dérègle l'épissage alternatif des pré-ARNm codant la troponine T rapide ( TNNT3) dans le muscle humain vaste externe. En outre, nous posons l'hypothèse selon laquelle des exercices contre résistance suffisent à eux seuls ou en combinaison avec la consommation d'acides aminés essentiels à atténuer les effets liés à l'âge sur l'épissage alternatif de TNNT3. D'après nos résultats, le vieillissement affecte négativement le mode d'épissage alternatif des pré-ARNm de la TNNT3 de façon à s'associer quantitativement à la diminution de la performance liée à l'âge. Curieusement, même si l'épissage alternatif de TNNT3 du vaste externe n'est pas altéré par une séance d'exercice contre résistance effectuée 24 h avant la biopsie musculaire, la consommation d'un mélange d'acides aminés essentiels à la suite de la séance d'exercice contre résistance entraîne une modification significative du mode d'expression par épissage de TNNT3 chez les deux groupes d'âge de façon à promouvoir une plus grande performance musculaire. En conclusion, la supplémentation en acides aminés essentiels après un exercice contre résistance pourrait constituer une stratégie pour diminuer les dérèglements des qualités musculosquelettiques au cours du vieillissement chez l'humain. [Traduit par la Rédaction] [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Applied Physiology, Nutrition & Metabolism is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
      2Department of Biology, The Pennsylvania State University, University Park, PA 16802, USA.
      3Department of Public Health Sciences, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
      4Department of Nutrition and Metabolism, University of Texas Medical Branch, Galveston, TX 77555, USA.
    • ISSN:
      1715-5312
    • Accession Number:
      10.1139/apnm-2014-0568
    • Accession Number:
      108741664
  • Citations
    • ABNT:
      COBLE, J. et al. Influence of ageing and essential amino acids on quantitative patterns of troponin T alternative splicing in human skeletal muscle. Applied Physiology, Nutrition & Metabolism, [s. l.], v. 40, n. 8, p. 788–796, 2015. DOI 10.1139/apnm-2014-0568. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=108741664&custid=s8280428. Acesso em: 29 maio. 2020.
    • AMA:
      Coble J, Schilder RJ, Berg A, Drummond MJ, Rasmussen BB, Kimball SR. Influence of ageing and essential amino acids on quantitative patterns of troponin T alternative splicing in human skeletal muscle. Applied Physiology, Nutrition & Metabolism. 2015;40(8):788-796. doi:10.1139/apnm-2014-0568.
    • APA:
      Coble, J., Schilder, R. J., Berg, A., Drummond, M. J., Rasmussen, B. B., & Kimball, S. R. (2015). Influence of ageing and essential amino acids on quantitative patterns of troponin T alternative splicing in human skeletal muscle. Applied Physiology, Nutrition & Metabolism, 40(8), 788–796. https://doi.org/10.1139/apnm-2014-0568
    • Chicago/Turabian: Author-Date:
      Coble, Joel, Rudolf J. Schilder, Arthur Berg, Micah J. Drummond, Blake B. Rasmussen, and Scot R. Kimball. 2015. “Influence of Ageing and Essential Amino Acids on Quantitative Patterns of Troponin T Alternative Splicing in Human Skeletal Muscle.” Applied Physiology, Nutrition & Metabolism 40 (8): 788–96. doi:10.1139/apnm-2014-0568.
    • Harvard:
      Coble, J. et al. (2015) ‘Influence of ageing and essential amino acids on quantitative patterns of troponin T alternative splicing in human skeletal muscle’, Applied Physiology, Nutrition & Metabolism, 40(8), pp. 788–796. doi: 10.1139/apnm-2014-0568.
    • Harvard: Australian:
      Coble, J, Schilder, RJ, Berg, A, Drummond, MJ, Rasmussen, BB & Kimball, SR 2015, ‘Influence of ageing and essential amino acids on quantitative patterns of troponin T alternative splicing in human skeletal muscle’, Applied Physiology, Nutrition & Metabolism, vol. 40, no. 8, pp. 788–796, viewed 29 May 2020, .
    • MLA:
      Coble, Joel, et al. “Influence of Ageing and Essential Amino Acids on Quantitative Patterns of Troponin T Alternative Splicing in Human Skeletal Muscle.” Applied Physiology, Nutrition & Metabolism, vol. 40, no. 8, Aug. 2015, pp. 788–796. EBSCOhost, doi:10.1139/apnm-2014-0568.
    • Chicago/Turabian: Humanities:
      Coble, Joel, Rudolf J. Schilder, Arthur Berg, Micah J. Drummond, Blake B. Rasmussen, and Scot R. Kimball. “Influence of Ageing and Essential Amino Acids on Quantitative Patterns of Troponin T Alternative Splicing in Human Skeletal Muscle.” Applied Physiology, Nutrition & Metabolism 40, no. 8 (August 2015): 788–96. doi:10.1139/apnm-2014-0568.
    • Vancouver/ICMJE:
      Coble J, Schilder RJ, Berg A, Drummond MJ, Rasmussen BB, Kimball SR. Influence of ageing and essential amino acids on quantitative patterns of troponin T alternative splicing in human skeletal muscle. Applied Physiology, Nutrition & Metabolism [Internet]. 2015 Aug [cited 2020 May 29];40(8):788–96. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=108741664&custid=s8280428